Objective To investigate the possibility of superparamagnetic iron-oxide(SPIO) particles as a marker of atherosclerotic plaques detected with 1.5T magnetic resonance imaging system in New Zealand white rabbit model.Methods Model of atherosclerosis were established by injuring the right carotid artery endothelium with balloon and feeding with high lipid diet.Five rabbits(group A) were intravenously injected SPIO;and the other five rabbits(group B) received the same volume of saline injection.The MR imaging was performed at 24,48 and 72 hours after injection,and then,histological study was done.Results Twenty-four hours after the intravenous injection of SPIO,3 rabbits in group A showed signal intensity loss in the right carotid artery wall on T2*WI,corresponding to Perl's Prussian blue staining-positive particles in the plaques revealed by histopathological study.T2*WI change and Perl's Prussian blue staining-positive particles in the plaques were not observed in any one model in group B.Conclusion It is possible to use superparamagnetic iron-oxide (SPIO) particles as a marker of atherosclerotic plaques detected with 1.5T magnetic resonance imaging system in New Zealand white rabbit model.
Objective To study the characteristics of interaction between the kaempferol(KF) and bovine serum albumin(BSA). Methods The quenching mechanism of the fluorescence of BSA by KF was studied with fluorescence spectra. The dynamic and static quenching constants were determined by the revised Stern-Volmer equation. The number of binding sites was calculated with double logarithmic equation and the main binding force was discussed by thermodynamic equations. The binding distance and energy transfer efficiency between donor(BSA) and acceptor(KF) were obtained based on Forster's nonradiative energy transfer theory. Results KF effectively quenched fluorescence of BSA via a combination of static and dynamic quenching processes. The binding constant KA was calculated to be in the order of 108, indicating a strong interaction between KF and BSA. The number of binding site is approximately equal to 2, the binding distance is 1.45 nm, the energy transfer efficiency is 0.73, and the binding force is mainly hydrophobic force. Conclusion KF effectively quenched the intrinsic fluorescence of BSA via combination of static and dynamic quenching mechanism, and the binding is mainly driven by the hydrophobic interaction.
WHO promotes evidence-based traditional medicine. Methodological issues limit evaluation of therapeutic effect of TCM. Current methods for evaluation do not properly reflect the humanity characteristics and holistic approach and differentiation of syndrome and prescription. TCM therapeutics is a complex intervention, and there is a limitation of methodology by only randomized, double-blind, placebo-controlled trials as it doesn't comply with bio-psycho-social-environmental and spiritual model for modern medicine. To fairly evaluate TCM therapeutic, we need to combine both quantitative and qualitative methods together, so as to create modality and methodology for TCM differentiation of syndrome and treatment.
Objective To compare the effects of recruitment maneuver in intra-and extra-pulmonary acute respiratory distress syndromes.Methods Fifty-three patients with ARDS who received recruitment maneuver(RM) were assigned into group ADRSp(n=25) and group ARDSexp(n=28) according to the causes of lung injury. All the patients were ventilated with tidal volume of 6 mL/kg of predicted body weight and limited plateau pressure less than 30 cmH2O. RMs were conducted by applying CPAP of 40 cmH2O for 40 s, repeated every 8 hours during the first 5 days. If the patient began weaning within the first 5 days, RMs were stopped . Both physiologic data and outcome measurements, including arterial blood gas(PaO2, PaCO2), airway pressure(peak pressure, plateau pressure), ventilator settings( tidal volume, PEEP, FiO2) , hemodynamic parameters, ICU-free days by day 28, ventilator-free days by day 28 and rate of survival with unassisted breathing by day 28, ICU and 28-day mortality, were compared between 2 groups.Results The PaO2/FiO2 increased after RM on day 1 and day 2(144.9 mmHg vs 128.0 mmHg, P=0.007, 205.0 mmHg vs 175.0 mmHg, P=0.001, respectively), accomparied by significant decrease in FiO2(0.58±0.16 vs 0.68±0.22, P=0.001;0.53±0.15 vs 0.54±0.15, P=0.017; respectively). But there was no significant difference in changes of PaO2/FiO2 and FiO2 between two groups, so was also PaCO2.During the first 5 days, no differences in peak pressure and plateau pressure between 2 groups were found. Each time when RM was conducted, no difference in heart rate and mean blood pressure before RM and 0.5, 1, 2, 4, 6-hours after RM were found. There were also no differences in ICU-free days, ventilator-free days, rate of survival with unassisted breathing, ICU and 28-day mortality between 2 groups. Only one patient was reported to suffer from low blood pressure when RM was performed. There were no incidence of barotraumas and other adverse effects.Conclusion RM is safe and effective in improving oxygenation in early ARDS. It has a beneficial impacts on the outcome. No significant differences are found in oxygenation, lung mechanics, hemodynamic parameters and prognosis between ARDSexp and ARDSp.
