正常人与慢性牙周炎患者牙槽骨组织培养细胞的生物学特性

首都医科大学学报 ›› 2006, Vol. 27 ›› Issue (4): 556-558.

正常人与慢性牙周炎患者牙槽骨组织培养细胞的生物学特性

佟丹¹, 陈世璋², 孙明学², 黄靖香³, 赵斌³

1. 首都医科大学附属北京友谊医院口腔科;2. 首都医科大学附属北京朝阳医院口腔科;3. 解放军总医院骨科研究所

收稿日期:2005-10-25 修回日期:1900-01-01 出版日期:2006-08-24 发布日期:2006-08-24

Biological Characteristics of Cell Lines Derived Form Human Dental Alveolus

Tong Dan¹, Chen Shizhang², Sun Mingxue², Huang Jingxiang³, Zhao Bin³

1. Department of Steomatology, Beijing Friendship Hospital, Capital University of Medical Sciences;2. Department of Steomatology, Beijing Chaoyang Hospital, Capital University of Medical Sciences;3. PLA General Hospital, Institute of Orthopedics

Received:2005-10-25 Revised:1900-01-01 Online:2006-08-24 Published:2006-08-24

摘要/Abstract

摘要： 目的比较正常人和慢性牙周炎患者的牙槽骨组织细胞系的生物学特性.方法原代培养牙周炎患者组(异常牙槽骨组)细胞系(H-171)和正常组细胞系(H-258和H-262),用组织化学、免疫组化等方法染色,观察细胞形态.用细胞计数法计算出2种细胞的倍增时间和分裂指数.结果异常牙槽骨组织原代培养一次成功,细胞形态为梭形.正常人牙槽骨组织原代细胞存活,生长速度较H-171慢.2组细胞均贴壁生长,具有成骨细胞的特点:碱性磷酸酶(AKP)染色、甲苯胺蓝、藩红花O、四环素标记矿化结节组织化学染色、过碘酸雪夫(PAS)、Ⅰ型胶原、BMP-2免疫组织化学染色均为阳性.结论 2组细胞均具有成骨细胞特点.生长速度H-171较H-258、H-262快.

关键词: 种子细胞系, 慢性牙周炎, 牙槽骨

Abstract: Objective To investigate the biological characteristics of cell lines established from healthy and diseased human dental alveoli.Methods We first established primary cell lines from either healthy or diseased or human dental alveolus.Two cell lines,H-258 and H-171,respectively derived from healthy or diseased tissues,were selected for further studies on cell morphology,growth and aging by cell counting,histochemical and immunohistochemical staining.Results Primary cell lines were successfully established from diseased human dental alveoli.Cell lines from healthy human dental alveoli were alive but with decreased growth speed.Both cell lines(H-171 and H-258) shared the characteristics with osteoblast as they showed an attached growth type and had positive staining with APK,tolduidine blue and BMP-2 ect.Conclusion Both cell lines shared the characteristics with osteoblast.Primary cell lines established from diseased human dental alveoli showed greater growth potential than that from healthy dental.

Key words: primary cell lines, chronic periodontitis, dental alveolus

中图分类号:

佟丹;陈世璋;孙明学;黄靖香;赵斌. 正常人与慢性牙周炎患者牙槽骨组织培养细胞的生物学特性[J]. 首都医科大学学报, 2006, 27(4): 556-558.

Tong Dan;Chen Shizhang;Sun Mingxue;Huang Jingxiang;Zhao Bin. Biological Characteristics of Cell Lines Derived Form Human Dental Alveolus[J]. Journal of Capital Medical University, 2006, 27(4): 556-558.

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