莪术醇及莪术醇磷酸酯单钠对Ehrlich腹水癌细胞膜电位和钾离子通透的影响

首都医科大学学报 ›› 1989, Vol. 10 ›› Issue (1): 23-27.

莪术醇及莪术醇磷酸酯单钠对Ehrlich腹水癌细胞膜电位和钾离子通透的影响

陈红¹, 邓德宁^1,2, 范茜¹

1. 北京医科大学物理学教研室;2. 首都医学院物理学教研室

收稿日期:1988-09-08 修回日期:1900-01-01 出版日期:1989-01-15 发布日期:1989-01-15

The Effects of Curcumol and Sodium Phosphorylcurcumol on The Membrane Potential and Membrane Permeability to Potassium Ions of Ehrlich Ascites Tumor Cells

Chen Hong¹

Department of Biomedical Engineering, Capital Institute of Medicine

Received:1988-09-08 Revised:1900-01-01 Online:1989-01-15 Published:1989-01-15

摘要/Abstract

摘要： 用荧光探剂dis-C₃-（5）作为跨膜电位指示剂并利用原子吸收光谱,观察莪术醇（curcumol）和莪术醇磷酸酯单纳（CP-Na）作用于Ehrlich腹水癌细胞引起的膜电位和膜对钾离子通透性的变化。莪术醇及CP-Na与癌细胞发生作用后,均可使dis-C₃-（5）的荧光强度增加。从valinomycin诱导的钾扩散电位与荧光强度变化的校正曲线可以看出,莪术醇使膜电位去极化约52mV。而CP-Na可使膜电位去极化约35mV。进一步实验证明莪术醇及CP-Na作用于细胞后均使细胞内钾离子浓度下降,相应的细胞外钾浓度上升。

关键词: 莪术醇, 抗癌物, 细胞膜电位, 艾氏腹水癌

Abstract: Curcumol is the antitumor constituent of the plant, Curcuma Wenyujin To study the effects of curcumol and sodium phosphorylcureumol （CP-Na） on the membrane of Ehrlich ascites tumor cells, fluoresent probe dis-C₃-（5） and atomic absorption spectrum techniques were used respectively to measure the changes in membrane potentied and membrane permeability to potassium ions. The intensity of fluorescence increases after addition of curcumol and CP-Na to suspension of tumor cells. The intensity of fluorescence was calibrated by means of potassium diffusion potential induced by valinomycin. By these methods the change of membrane potential can be calculated, for curcumol and CP-Na mem- brane potential of tumor cells was deporlarigzed about 52mV a and 35mV. The intracellular potassium ion concentration of tumor cells decreases after adding the curcumol and CP-Na, and potassium ion coucentration Within the medium increases. The mechanisms of those changes mentioned above were cliscussea.

Key words: curcumol, antitumor agent, cell membrane potential, Ehrlich ascites tumor

陈红;邓德宁;范茜. 莪术醇及莪术醇磷酸酯单钠对Ehrlich腹水癌细胞膜电位和钾离子通透的影响[J]. 首都医科大学学报, 1989, 10(1): 23-27.

Chen Hong. The Effects of Curcumol and Sodium Phosphorylcurcumol on The Membrane Potential and Membrane Permeability to Potassium Ions of Ehrlich Ascites Tumor Cells[J]. Journal of Capital Medical University, 1989, 10(1): 23-27.