泡球蚴组织及囊液可溶性蛋白质组分和抗原初步分析

首都医科大学学报 ›› 1991, Vol. 12 ›› Issue (4): 253-256.

泡球蚴组织及囊液可溶性蛋白质组分和抗原初步分析

孙铁, 姜洪杰, 陈佩惠, 杨连雪

首都医学院寄生虫学教研室

收稿日期:1991-02-23 修回日期:1900-01-01 出版日期:1991-10-15 发布日期:1991-10-15

Preliminary Analyses on the Soluble Proteins and Antigens of Alveolar Hydatid Tissues and Cyst Fluid

Sun Tie, Jiang Hongjie, Chen Peihui, Yang Lianxue

Capital Institute of Medicine

Received:1991-02-23 Revised:1900-01-01 Online:1991-10-15 Published:1991-10-15

摘要/Abstract

摘要： 应用SDS-PAGE分析了泡球蚴囊液及全囊、原头蚴和囊壁组织可溶性蛋白质,结果分别出现23、26、16、25条蛋白带,其中主带分别为4、7、5、7条。用斑点ELISA显示,4种抗原的敏感性分别为67%、67%、100%、67%,特异性分别为90%、89%、83.5%、91%。

关键词: 泡球蚴, 可溶性蛋白质, SDS-PAGE, 斑点 ELISA

Abstract: The soluble proteins of cyst fluid,hydatid cysts,protoscoleces and cyst walls of alveolar hydatid obtained from experimentally infected gerbils(Meriones unguiculatus)were studied with SDS-PAGE.Four specimens showed respectively 23,26,26,and 25 protein bands with their major bands being respectively 4,7,5,and 7.Four kinds of soluble proteins mentioned above were tested as antigens with Dot-ELISA.Asensitivity of 67%,67%,100%, and 67% were found while the specificity showed 90%,89%,83.5%,and 91% respectively.

Key words: alveolar hydatid, soluble protein, SDS-PAGE, Dot-ELISA

孙铁;姜洪杰;陈佩惠;杨连雪. 泡球蚴组织及囊液可溶性蛋白质组分和抗原初步分析[J]. 首都医科大学学报, 1991, 12(4): 253-256.

Sun Tie;Jiang Hongjie;Chen Peihui;Yang Lianxue. Preliminary Analyses on the Soluble Proteins and Antigens of Alveolar Hydatid Tissues and Cyst Fluid[J]. Journal of Capital Medical University, 1991, 12(4): 253-256.

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