猪囊虫头节、囊壁抗原Dot-ELISA和IEST试验在脑囊虫病诊断中的初步比较研究

首都医科大学学报 ›› 1991, Vol. 12 ›› Issue (1): 7-9.

猪囊虫头节、囊壁抗原Dot-ELISA和IEST试验在脑囊虫病诊断中的初步比较研究

姜洪杰¹, 卞英华¹, 贾秋英², 杨连雪¹, 王凤芸¹

1. 首都医学院寄生虫学教研室;2. 东单三条儿童医院

收稿日期:1989-12-06 修回日期:1900-01-01 出版日期:1991-01-15 发布日期:1991-01-15

A Comparative Study on the Diagnosis for Neurocysticercosis by CSAg,CWAg Dot-ELISA and Immunoenzymatic Staining Technique(IEST)with Cysticerus Cellulosae Frozen Section as Antigens

Jiang Hongjie¹, Bian Yiughua¹, Jia Qiuying², Wang Feugyun¹

Department of Parasitology, Capital Institute of Medicine

Received:1989-12-06 Revised:1900-01-01 Online:1991-01-15 Published:1991-01-15

摘要/Abstract

摘要： 比较了猪囊虫头节抗原(CSAg)、囊壁抗原(CWAg)斑点(ELISA)和免疫酶染色试验(IEST)在脑囊虫病诊断中的敏感性、特异性和交叉反应.CSAg、CWAgDot-ELISA的检出阳性率分别是94.6%(53/56)和96.4%(54/56),IEST的阳性率为98.2%.两种方法与正常人、并殖吸虫病和华支睾吸虫病患者血清均无假阳性反应、但与细粒棘球蚴病患者血清均有交叉反应.

关键词: 猪囊虫头节抗原, 囊壁抗原, 斑点 ELISA, 免疫酶染色试验

Abstract: In this paper,the results of CSAg Dot-ELISA and IEST with frozen section of cysticerus cellulosae as antigesn in the diagnosis neurocysticercosis were compared.Positive rales of CSAg,CWAg Dot-ELISAand IESTwere 94.6% (53/56),96.4% (54/56) and 98.2%(55/56) respectively.No cross-reaction was observed with sera from cases oFconorchiasis and paragonimiasis,while none of the 50 serum samples from normal controls was found positive.CSAg,CWAg Dot-ELISA and IEST with serum specimens from echinococcosis were 40.9% (9/22),22.7%(5/22) and 18.1%(4/22) respectively.The results showed both Dot-ELISA and IEST with frozen sections as antigens in the diagnosis of neurocysticercosis were considered to be of higher sensitivity and specificity.

Key words: antigen of scolex of cysticerus cellulosae, an igen of cyst wall, Dot-ELISA, IEST

姜洪杰;卞英华;贾秋英;杨连雪;王凤芸. 猪囊虫头节、囊壁抗原Dot-ELISA和IEST试验在脑囊虫病诊断中的初步比较研究[J]. 首都医科大学学报, 1991, 12(1): 7-9.

Jiang Hongjie;Bian Yiughua;Jia Qiuying;Wang Feugyun. A Comparative Study on the Diagnosis for Neurocysticercosis by CSAg,CWAg Dot-ELISA and Immunoenzymatic Staining Technique(IEST)with Cysticerus Cellulosae Frozen Section as Antigens[J]. Journal of Capital Medical University, 1991, 12(1): 7-9.

[1]	孙铁;姜洪杰;陈佩惠;杨连雪. 泡球蚴组织及囊液可溶性蛋白质组分和抗原初步分析[J]. 首都医科大学学报, 1991, 12(4): 253-256.
[2]	姜洪杰;贾秋英;杨连雪;卞英华. 应用猪囊尾蚴囊液、头节和囊壁抗原斑点ELISA诊断脑囊虫病的比较研究[J]. 首都医科大学学报, 1991, 12(2): 114-116.