羊心浦肯野纤维钠泵活动与动作电位长度关系

首都医科大学学报 ›› 1991, Vol. 12 ›› Issue (2): 98-104.

羊心浦肯野纤维钠泵活动与动作电位长度关系

韩大英

首都医学院心血管生理研究室

收稿日期:1990-11-30 修回日期:1900-01-01 出版日期:1991-04-15 发布日期:1991-04-15

On the Relationship Between Sodium—Pump and Action Potential Duration in Cardiac Purkinje Fibres

Han Daying

Department of Cardiovasculat Physiology Capital Institute of Medicine

Received:1990-11-30 Revised:1900-01-01 Online:1991-04-15 Published:1991-04-15

摘要/Abstract

摘要： 采用羊心浦肯野纤维标本,间断地施以快速刺激,用普通玻璃微电极与离子选择电极同时记录细胞动作电位(AP)、细胞内Na⁺活度(a_Naⁱ)及细胞间K⁺活度(a_k⁰).或者,在固定频率刺激下将标本短时间置于无K⁺液中作预处理,以提高a_Naⁱ,刺激钠泵活动.目的在于观察细胞膜钠泵活动及跨膜被动K⁺流在APD变化中的调控作用.结果表明,在心率的生理范围内(60～150次/min),致电钠泵电流在总复极电流中占12%～15%。

关键词: 浦肯野纤维, 离子选择电极, 钠泵, K⁺流, 动作电位

Abstract: Measurements of the intracellular sodium activity(a_Naⁱ,as a indicator of sodium-pump activity)and the paracellular potassium activity(a_K⁰)were performed in sheep cardiac Purkinje fibres at rest and during stimulation using ion-selective microelectrodes.During a period of stimulation,paracellular potassium first increased and then declined below control values as long as pump activity was enhanced.The time course of the maximal diastolic potential was similar to pump activity,whereas that of the action potential duration deviated markedly. Further experiments on the effect of pump activation following challenges to zero potassium solution revealed that the rate dependence of the action potential duration was more sensitive to extracellular calcium than it was to the pump activity.Quantitative analysis of the data showed that in the physiological range of heart rate(60～150/min),the contribution of the electrogenic pump current to total repolarizing currents is between 12% and 25%.

Key words: Purkinje fibres, ion-selective microelectrode, sodium pump, K⁺current, action potential

韩大英. 羊心浦肯野纤维钠泵活动与动作电位长度关系[J]. 首都医科大学学报, 1991, 12(2): 98-104.

Han Daying. On the Relationship Between Sodium—Pump and Action Potential Duration in Cardiac Purkinje Fibres[J]. Journal of Capital Medical University, 1991, 12(2): 98-104.

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