应用猪囊尾蚴囊液、头节和囊壁抗原斑点ELISA诊断脑囊虫病的比较研究

首都医科大学学报 ›› 1991, Vol. 12 ›› Issue (2): 114-116.

应用猪囊尾蚴囊液、头节和囊壁抗原斑点ELISA诊断脑囊虫病的比较研究

姜洪杰¹, 贾秋英², 杨连雪¹, 卞英华¹

1. 首都医学院寄生虫学教研室;2. 北京东单三条儿童医院

收稿日期:1990-08-07 修回日期:1900-01-01 出版日期:1991-04-15 发布日期:1991-04-15

A Comparative Study on the Diagnosis in Neurocysticercosis with CFAg, CSAg and CWAg Dot-ELISA of Cysticerus Cellulosae

Jiang Hongjie¹, Jia Qiuying², Yang Lianxue¹, Bian Yinghua¹

Department of Parasitology, Capital Institute of Medicine

Received:1990-08-07 Revised:1900-01-01 Online:1991-04-15 Published:1991-04-15

摘要/Abstract

摘要： 应用猪囊尾蚴囊液、头节和囊壁抗原斑点ELISA检测脑囊虫病患者血清.当血清稀释度为1:20～1:320时,囊液、头节和囊壁抗原对脑囊虫病患者血清的检出阳性率分别为95:7%～82.9%,95.7%～71.4%和97.1%～30%。3种抗原与正常人、肺吸虫病人、华支睾吸虫病人及脑血管病人血清均无假阳性反应或交叉反应。与细胞粒棘球蚴病人血清的交叉反应率分别是22.5%～47.5%,10%～42.5%和10%～17.5%,以囊壁抗原的交叉反应为最低。

关键词: CF抗原, CS抗原, CW抗原, 斑点 ELISA

Abstract: This article deals with the diagnostic values,sensitivity and specificity of Dot-ELISA in neurocysticercosis cases with cyst fluilds(CFAg),scolex(CSAg)and cyst wall(CWAg)of Cysticerus cellulosae as antigens.Positive rates of Dot-ELISA with these 3 kinds of antigens for neurocysticercosis(70 cases)were 95.7%～82.9,95.7～71.4% and 97.1～30% respectively in dilution of examined sera ranged from 1:20 to 1:320.There was no cross-reaction in subjects with normal controls(50 cases),clonorchiasis(50 cases),paragonimiasis (30 cases)and cerebrovascular disease(20 cases).Sera from 40 cases of echinococcosis patients showed positive reaction with these 3 antigens,with positive rates of 22.5～ 47.5%,10%～42.5% and 10%～17.5% respectively in different dilution of the examined sera,however the rate of cross-reaction with CWAg was lower than the other two antigens.

Key words: CFAg, CSAg, CWAg, Dot-ELISA

姜洪杰;贾秋英;杨连雪;卞英华. 应用猪囊尾蚴囊液、头节和囊壁抗原斑点ELISA诊断脑囊虫病的比较研究[J]. 首都医科大学学报, 1991, 12(2): 114-116.

Jiang Hongjie;Jia Qiuying;Yang Lianxue;Bian Yinghua. A Comparative Study on the Diagnosis in Neurocysticercosis with CFAg, CSAg and CWAg Dot-ELISA of Cysticerus Cellulosae[J]. Journal of Capital Medical University, 1991, 12(2): 114-116.

[1]	孙铁;姜洪杰;陈佩惠;杨连雪. 泡球蚴组织及囊液可溶性蛋白质组分和抗原初步分析[J]. 首都医科大学学报, 1991, 12(4): 253-256.
[2]	姜洪杰;卞英华;贾秋英;杨连雪;王凤芸. 猪囊虫头节、囊壁抗原Dot-ELISA和IEST试验在脑囊虫病诊断中的初步比较研究[J]. 首都医科大学学报, 1991, 12(1): 7-9.