用生物发光技术对结核杆菌H37Ra株进行快速药物敏感试验的研究

首都医科大学学报 ›› 1990, Vol. 11 ›› Issue (2): 105-108.

用生物发光技术对结核杆菌H₃₇Ra株进行快速药物敏感试验的研究

沈海中¹, 安云庆¹, 李玉兰¹, 程松高¹, 单菊生², 莫家秀², 郭均²

1. 首都医学院微生物学教研室;2. 北京市结核病肺部肿瘤研究所细菌免疫室

收稿日期:1989-07-26 修回日期:1900-01-01 出版日期:1990-04-15 发布日期:1990-04-15

The Study of Rapid Drug Sensitivity of M. tuberculosis Strain H₃₇ Ra with Bioluminescence

Shen Haizhong¹, An Yunqing¹, Li Yulan¹, Cheng Songgao¹, Shan Jusheng², Mo Jiaxiu², Guo Jun²

1. Department of Microbiology, Capital Institute of Medicine;2. Institute of Tuberculosis and Lung Cancer, Beijing

Received:1989-07-26 Revised:1900-01-01 Online:1990-04-15 Published:1990-04-15

摘要/Abstract

摘要： 用生物发光技术对结核杆菌H₃₇Ra株进行快速药物敏感试验的研究。该法系将在改良罗氏培养基中培养3周的结核杆菌H₃₇Ra株经适当稀释后,移种于0.1%吐温80-综合苏通液体培养基中(加与未加抗生素),37℃培养4～5d,然后测定细菌ATP提取物BL(305)值。通过计算细菌生长抑制率,了解细菌对药物的敏感性.本法快速(4～5d),易于操作。

关键词: 生物发光, 链霉素, 结核杆菌

Abstract: We carried out studies of rapid drug sensitivity testing of M. tuberculosis strain H₃₇Ra with the bioluminescence method. The method was that bacteria from about three weeks old cultures of M. tuberculosis strain H₃₇Ra on Lowenstein Jensen medium were diluted suitably and were added to 0.1 per cent Tween 80--system Sauton liquid medium (with or without antibiotics and free medium) and then incubated at 37℃ After inculation for four or five days, the amout of BL_30s rlu＇s were determined. We can know the drug sensitivity of M. tuberculosis strain H₃₇Ra which was estimated according to the inhibition rate of bacteria growth. The results of the seusitivity testing can be evaluated within 4 to 5 days. The method is rapid and easy to perform.

Key words: bioluminescence, streptomycin, M. tuberculosis

沈海中;安云庆;李玉兰;程松高;单菊生;莫家秀;郭均. 用生物发光技术对结核杆菌H₃₇Ra株进行快速药物敏感试验的研究[J]. 首都医科大学学报, 1990, 11(2): 105-108.

Shen Haizhong;An Yunqing;Li Yulan;Cheng Songgao;Shan Jusheng;Mo Jiaxiu;Guo Jun. The Study of Rapid Drug Sensitivity of M. tuberculosis Strain H₃₇ Ra with Bioluminescence[J]. Journal of Capital Medical University, 1990, 11(2): 105-108.

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用生物发光技术对结核杆菌H₃₇Ra株进行快速药物敏感试验的研究

The Study of Rapid Drug Sensitivity of M. tuberculosis Strain H₃₇ Ra with Bioluminescence

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