羊心脏浦肯野纤维膜钠泵活动的测量

首都医科大学学报 ›› 1992, Vol. 13 ›› Issue (1): 1-7.

羊心脏浦肯野纤维膜钠泵活动的测量

韩大英, 刘北英

首都医学院心血管生理研究室

收稿日期:1990-12-29 修回日期:1900-01-01 出版日期:1992-01-15 发布日期:1992-01-15

Measurement of Sodium Pump Activity in Sheep Cardiac Purkinje Fibres

Han Daying, Liu Beiying

Department of Cardiovascular Physiology, Capital Institute of Medicine

Received:1990-12-29 Revised:1900-01-01 Online:1992-01-15 Published:1992-01-15

摘要/Abstract

摘要： 采用细胞内Na⁺选择电极方法,先以不同频率电刺激驱动羊心脏浦肯野纤维标本,使细胞内Na⁺活度(aⁱN+a)上升到不同高度,在停搏即刻测量Na⁺逐出速率(d(a_Naⁱ)/dt)。进而将所测得d(a_Naⁱ)/dt值相对于各时刻a_Naⁱ作相关曲线(SP-IS:Sodium Pump-Internal Sodium),比较其斜率可以了解膜钠泵活动的相对强弱。这种方法可以较迅速、简便地在生理状态下反复测算膜钠泵活动速率。进而用外差法找到相关曲线与纵坐标交点(Na_ia⁺),它反应了静息态时Na⁺内流速率。还观察了pH值变化、高或低K⁺,高或低Ca²⁺及Mn²⁺、异丙基肾上腺素等对膜钠泵活动速率的影响。

关键词: 细胞内Na⁺活度, 钠泵活动, 离子选择电极, 浦肯野纤维, 异丙基肾上腺素

Abstract: Raising the internal sodium ion activity (a_Naⁱ) by way of intermittent fast beating the sheep cardiac Purkinje fibers in different frequencies resulted in declining of a_Naⁱ at the very end of the train of beats.Both of a_Naⁱ increments and declining rates (d(a_Naⁱ/dt) paralleled with the stimulation frequencies.The values of d(a_Naⁱ)/dt were plotted against the corre-sponding a_Naⁱ to obtain a correlation curve (sodium pump-internal sodium relationship, SP-IS).Sodium pump activity can be evaluated by comparing the slopes of correlation curves.The intersects of the correlation curves and the ordinate (Vmax) was got with extrapola-tion.The sum of values of Vmax and the maximum d(a_Naⁱ)/dt represented the maximum ac-tivity of sodium pump.The pump activity can be calculated simply and repeatedly with this method.The effects of high/low pH, K⁺, Ca²⁺, and 4 mmol/L Mn²⁺, 5×10^-7mol/L iso-prenaline on pump activity were observed simultaneously.

Key words: internal sodium ion activity, sodium pump activity, ion-selective microelectrode, Purkinje fibre, isoprenaline

中图分类号:

Q422

韩大英;刘北英. 羊心脏浦肯野纤维膜钠泵活动的测量[J]. 首都医科大学学报, 1992, 13(1): 1-7.

Han Daying;Liu Beiying. Measurement of Sodium Pump Activity in Sheep Cardiac Purkinje Fibres[J]. Journal of Capital Medical University, 1992, 13(1): 1-7.