我国4个旋毛虫分离株DNA限制性酶切片断长度差异的比较研究

首都医科大学学报 ›› 1995, Vol. 16 ›› Issue (1): 19-23.

我国4个旋毛虫分离株DNA限制性酶切片断长度差异的比较研究

何忠平¹, 陈佩惠¹, 卢思奇¹, 赫翠珍²

1. 首都医学院寄生虫学教研室;2. 首都医学院分子生物学研究室

收稿日期:1994-06-20 修回日期:1900-01-01 出版日期:1995-01-15 发布日期:1995-01-15

Restriction Fragment Length Differences (RFLDs) Analysis of DNA Isolates of Trichinella spiralis from China

He Zhongping¹, Chen Peihui¹, Lu Siqi¹, He Cuizhen²

Department of Parasitology, Capital Institute of Medicine

Received:1994-06-20 Revised:1900-01-01 Online:1995-01-15 Published:1995-01-15

摘要/Abstract

摘要：

报告我国云南、河南、沈阳(猪)以及长春(犬)4个旋毛虫分离株,用10种限制性内切酶分析比较其基因组DNA酶切片断长度差异。结果表明:来自猪体与来自犬体的旋毛虫酶切图谱不同,显示宿主的不同比地理位置、气候条件差异在旋毛虫的分类上更有意义。

关键词: 旋毛虫, 基因组DNA, 限制性酶切片断长度差异

Abstract:

The restriction fragment length differences (RFLDs) of 4 domestic isolates of Trichinella spiralis from Yunnan, Henan, Shenyang, Changchun of China, were analysed. Trichinella spiralis of Yunnan, Henan, Shenyang were isolated from pig, while Trichinella spiralis of Changchun was isolated from dog. Total genomic DNA of muscle larve of each isolates was extracted with phonol/ethanol methods and digested separately with 10 restriction enzymes (BamHI,Dral,EcoRI,Hae Ⅲ, Hind Ⅲ, Hpa Ⅱ, Pst I, Pvu Ⅱ, XbaⅠ, XhoⅠ). All the DNA digested with enzymes were then carried on agarose gel electrophoresis for RFLDs analysis.The results shown that pattern differences were observed between isolates from pig and dog,and differences of host was useful more than differences of geography and climate in the taxonomy of Trichinella spiralis.

Key words: Trichinella spiralis, genomic DNA, restriction fragment length differences (RFLDs)

中图分类号:

R383.15

何忠平;陈佩惠;卢思奇;赫翠珍. 我国4个旋毛虫分离株DNA限制性酶切片断长度差异的比较研究[J]. 首都医科大学学报, 1995, 16(1): 19-23.

He Zhongping;Chen Peihui;Lu Siqi;He Cuizhen. Restriction Fragment Length Differences (RFLDs) Analysis of DNA Isolates of Trichinella spiralis from China[J]. Journal of Capital Medical University, 1995, 16(1): 19-23.

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