首都医科大学学报 ›› 1995, Vol. 16 ›› Issue (1): 27-31.

• 论著 • 上一篇    下一篇

小鼠生后睾丸发育的形态学与组织化学研究

史小林1, 诸定寿1, 路欣1, 马小蕊1, 许晴1, 张亚滨1, 孟庆鸣2   

  1. 1. 首都医学院生殖医学研究室;2. 北京市卫生学校
  • 收稿日期:1993-10-29 修回日期:1900-01-01 出版日期:1995-01-15 发布日期:1995-01-15

Morphologyical and Histochemical Studies on the Postnatal Development of Mice Testes

Shi Xianlin1, Zhi Dingshou1, Lu xin1, Ma Xiaorui1, Xu Qing1, Zhang Yabin1, Meng Qingming2   

  1. 1. Department of Reproductive Medical Research, Capital Institute of Medicne;2. Beijing Health School
  • Received:1993-10-29 Revised:1900-01-01 Online:1995-01-15 Published:1995-01-15

摘要:

对小鼠生后的发育进行了研究。形态学研究证明其发育可分为3个阶段:第1阶段为静止期(生后第1周),曲细精管为单层细胞构成的实心团。第2阶段为增殖、分化期(生后第2~4周),曲细精管上皮由单层变为复层,管壁由精原细胞、精母细胞、精子细胞及少量精子构成,附睾内出现少量精子。第3阶段为性成熟期(生后第5周之后),此期曲细精管内和附睾内都含有大量精子。组织化学研究发现第1周碱性磷酸酶(ALP)与氨基肽酶(AP),定位于曲细精管上皮细胞呈中等阳性;第2周上皮细胞内阳性减弱;第3周后上皮细胞内为阴性,而小管周围出现一层强阳性反应结构。通过本研究证明精子形成与性成熟是两个概念,并发现ALP及AP与睾丸的发育有密切关系。

关键词: 小鼠睾丸, 睾丸生后发育, 形态学, 组织化学

Abstract:

In this study, we have demostrated that there were three stages in the development of mice testes after birth, during the first week, the seminiferous tubules consisted of simple genetic epithelium that was in a state of relative rest. From the second tothe fourth week, the epithelium of seminiferous tubules proliferated into more layers, including sperma togonium,spermatocytes, and spermatids, and a few spermatozoa were found in the epididymis. Afterthe fifth week many spermatozoa were found in tubules of epididymies. We thought this stage presented sex maturity of mice. Alkaline phosphatase and aminopeptidase localized in epithelium of seminiferous tubules in first week. From the second week, the positive reactivity became weak or negative. However, after the end of the second week, these enzymes could be found around a tissue layer of the seminiferous tubules. We would study further the reason for these changes.

Key words: mice testes, postnatal development of testes, morphology histochemistry

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