关键词: 聚合酶链式反应, 序列特异性引物, 基因分型

Abstract: In order to select donor and recipient,accurate typing HLAespecially typing HLA-DR is one of the most important factor for a longer survival time of the graft.Using polymerase chain reaction with sequence specific primers(PCR-SSP)technique the authors underwent HLA-DR gene typing in 331 cases of donor and recipient. Among them,109 cases were subjected to a comparative study with serological typing. The results showed that the error rate of serological assay was 29.7%, while the HLA-DRgene typing with PCR-SSP method all showed their specificities without any false negative or false positive.Replication experiments in 20 cases of random sampling showed 100% coincidence. The typing results were confirmed by INNO-LIPA PCR-Reverse SSOexperiments,and the coincidence was 100%. Only 4 hours will suffice for this assay. So, it is simple, rapid and reliable as compared with the serological method, and is suitable for HLAtyping in clinical organ transplantation.

Key words: polymerase chain reaction(PCR), sequence specific primers(SSP), gene typing