CSF延长慢性粒细胞白血病病理克隆体外培养存活期限作用的研究

首都医科大学学报 ›› 1997, Vol. 18 ›› Issue (3): 235-237.

CSF延长慢性粒细胞白血病病理克隆体外培养存活期限作用的研究

徐娟, 孙雪静, 杨立, 田丁

首都医科大学宣武医院血液科

收稿日期:1997-04-16 修回日期:1900-01-01 出版日期:1997-07-15 发布日期:1997-07-15

Colony Stimulating Factor May Prolong Survival Time of Malignant Cells of Chronic Myelogenous Leukemia in Vitro

Xu Juan, Sun Xuejing, Yang Li, Tian Ding

Department of Hematology, Xuanwu Hospital, Affiliate of Capital University of Medical Sciences

Received:1997-04-16 Revised:1900-01-01 Online:1997-07-15 Published:1997-07-15

摘要/Abstract

摘要： 为观察集落刺激因子(CSF)对慢性粒细胞白血病(CML)病理克隆增殖的影响,采用单抗免疫磁珠二步分离法,从CML患者骨髓标本分离出带有恶性克隆标记的CD34⁺HLADR⁺细胞,并在体外观察干细胞因子(SCF)、白细胞介素-3(IL-3)和促红细胞生成素(Epo)联合应用对细胞增殖的影响。结果：CSF存在体系与无CSF体系相比CML病理克隆消失延迟14d(P<0.05).结果提示：CSF具有延长CML病理细胞在体外培养存活期限的作用。

关键词: 慢性粒细胞白血病, 体外培养, 集落刺激因子

Abstract: In order to observe the effect of colony stimulating factor on proliferation of malignant cells of chronic myelogenous leukemia (CML) CD34⁺HLA DR⁺ cells which contain ph 1 chromosome and BCR/ABLrearrangement were separated from 8 CMLbone marrow sample by two step MoAb magnetical microbeads separating method,and the effect of colony stimulating factor(SCF IL-3 and Epo)on proliferation of this kind of cells were observed in vitro. The results indicated that elimination of malignant cells was delayed for 14 d in colony stimulating factor liquid culture as compared with liquid culture without colony stimulating factor(P<0.05).This observation indicates that colony stimulating factor may prolong the survival time of malignant cells of CMLin vitro.

Key words: chronic myelogenous leukemia, culture in vitro, colony stimulating factor

中图分类号:

R733.72

徐娟;孙雪静;杨立;田丁. CSF延长慢性粒细胞白血病病理克隆体外培养存活期限作用的研究[J]. 首都医科大学学报, 1997, 18(3): 235-237.

Xu Juan;Sun Xuejing;Yang Li;Tian Ding. Colony Stimulating Factor May Prolong Survival Time of Malignant Cells of Chronic Myelogenous Leukemia in Vitro[J]. Journal of Capital Medical University, 1997, 18(3): 235-237.

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