HPLC测定5-FU方法中内标物对氨基苯甲酸的研究

首都医科大学学报 ›› 2003, Vol. 24 ›› Issue (3): 237-239.

HPLC测定5-FU方法中内标物对氨基苯甲酸的研究

王桥, 宋学英, 张枫, 朱莹

首都医科大学化学教研室

收稿日期:2003-01-03 修回日期:1900-01-01 出版日期:2003-07-15 发布日期:2003-07-15

PABA as Internal Standard in Method of HPLC for Determining 5-FU Concentration in Serum

Wang Qiao, Song Xunying, Zhang Feng, Zhu Ying

Department of Chemistry, Capital University of Medical Sciences

Received:2003-01-03 Revised:1900-01-01 Online:2003-07-15 Published:2003-07-15

摘要/Abstract

摘要： 为改进HPLC测定氟尿嘧啶(5-fluorouracil,5-FU)的方法,选择对氨基苯甲酸(PABA)为内标物,用C₁₈色谱柱,流动相甲醇磷酸缓冲液(pH 6.8),检测波长270nm,测定血清中5-FU含量。结果5-FU的最小检出限量为1.2ng,5-FU及PABA的萃取回收率分别大于88.01%和86.55%。

关键词: 高效液相色谱法, 氟尿嘧啶, 对氨基苯甲酸

Abstract: To improve the determination of 5-FU by high-performance liquid chromatography, PABA was selected as an internal standard in the analytical method. For determination of 5-FU concentration in serum, a reversed-phase C₁₈ column with a mobile phase consisting methanol-phosphate buffer (pH 6.8) was used. UV detector was set at 270 nm. The limit of determination for 5-FU was 1.2ng. The extraction recovery of 5-FU and PABA were more than 88.1% and 86.55% respectively.

Key words: HPLC, 5-fluorouracil, p-aminobenzonic acid(PABA)

中图分类号:

R917
R927.2

王桥;宋学英;张枫;朱莹. HPLC测定5-FU方法中内标物对氨基苯甲酸的研究[J]. 首都医科大学学报, 2003, 24(3): 237-239.

Wang Qiao;Song Xunying;Zhang Feng;Zhu Ying. PABA as Internal Standard in Method of HPLC for Determining 5-FU Concentration in Serum[J]. Journal of Capital Medical University, 2003, 24(3): 237-239.

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