首都医科大学学报 ›› 2015, Vol. 36 ›› Issue (1): 127-131.doi: 10.3969/j.issn.1006-7795.2015.01.024

• 基础研究 • 上一篇    下一篇

慢性不可预见性应激对大鼠神经递质及下丘脑-垂体-肾上腺皮质轴的影响

杨亦龙, 姚晓泉, 王雅丽, 王海征, 欧阳俊摇, 刘思思, 王蕾, 张秋霞, 赵晖   

  1. 首都医科大学中医药学院中医临床基础学系 中医络病研究北京市重点实验室, 北京 100069
  • 收稿日期:2014-05-14 出版日期:2015-02-21 发布日期:2015-01-31
  • 通讯作者: 张秋霞, 赵晖 E-mail:zqx26@163.com;zhaohui8957@sina.com
  • 基金资助:
    北京市教委基金(KM201310025013);北京市属高等学校高层次人才引进与培养计划-长城学者项目资助(CIT&TCD20140329).

Effect of chronicunpredictable stress on neurotransmitter and hypothalamic-pituitary-adrenal axis in rats

Yang Yilong, Yao Xiaoquan, Wang Yali, Wang Haizheng, Ouyang Junyao, Liu Sisi, Wang Lei, Zhang Qiuxia, Zhao Hui   

  1. Department Traditional Chinese Medicine Clinical Basis, School of Traditional Chinese Medicine, Capital Medical University, Beijing Key Laboratory of TMC Collateral Disease Theory Research, Beijing 100069, China
  • Received:2014-05-14 Online:2015-02-21 Published:2015-01-31
  • Supported by:
    This study was supported by the Fund of Commission of Education of Beijing(KM201310025013); Program for Changcheng Scholars of the Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions (CIT&TCD20140329).

摘要: 目的 探讨慢性不可预见性应激(chronic unpredictable stress, CUS) 对大鼠神经递质和下丘脑-垂体-肾上腺皮质轴(hypothalamic-pituitary-adrenal axis, HPA)的影响.方法 16只SD雄性大鼠随机数字表法分为正常对照组和模型组.模型组大鼠给予慢性不可预见性的应激,检测其体质量增长量;通过蔗糖偏嗜实验观察大鼠行为学的改变;采用高效液相-电化学法测定海马多巴胺(dopamine, DA)、5-羟色胺(5-hydroxytryptamine, 5-HT),高效液相-荧光法测定谷氨酸(glutamic acid, Glu)、γ-氨基丁酸(γ-aminobutyric acid, γ-GABA);用放射免疫法检测血浆及下丘脑促肾上腺皮质激素释放激素(corticotropin-releasing hormone,CRH)、促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)、皮质酮(corticosterone,CORT).结果 与正常对照组相比,模型组大鼠体质量增长量显著降低,糖水消耗量明显下降,DA、γ-GABA显著降低,5-HT、Glu有升高的趋势,ACTH显著降低,CORT质量浓度明显增高.结论 CUS会导致大鼠行为学的改变,HPA轴的紊乱.

关键词: 慢性不可预见性应激, 行为学, 神经递质, 下丘脑-垂体-肾上腺皮质轴

Abstract: Objective To investigate the effect of chronic unpredictable stress (CUS) on neurotransmitter and hypothalamic-pituitary-adrenal axis(HPA)in rats. Methods Totally 16 male SD rats were randomly divided into normal group and model group. The model group was established by chronic unpredictable stress. The weights of rats were examined every day.The behavior changes of rats were observed by the sucrose preference test. The contents of dopamine(DA), 5-hydroxeytryptamine(5-HT)inhippocampus were measured by High performance liquid-electrochemical method. And the secretion of glutamic acid(Glu), γ-aminobutyric acid(γ-GABA) in hippocampus were determined by the method of high-effective liquid chromatography fluorescence.The concentrations of corticotropin-releasing hormone(CRH), adrenocorticotropic hormone (ACTH), corticosterone (CORT) in plasma and hypothalamus were tested by Radioimmunoassay(RIA)method. Results Compared with the normal group, the model group's weight was significantly reduced. The consumption of sucrose was decreased in model group. The contents of DA, γ-GABA in hippocampus was significantly reduced in model group, and the 5-HT, Glu had the trend of increase.The adrenocorticotropic hormone(ACTH) in hypothalamus was significantly decreased and the plasma's corticosterone(CORT) was significantly increased in model group.Conclusion The chronic unpredictable stress (CUS) could induce the changes of animal behavior and abnormalities of neurotransmitter and hypothalamic pituitary adrenal (HPA)axis.

Key words: chronic-unpredictable-stress, behavior, neurotransmitter, hypothalamic-pituitary-adrenal axis

中图分类号: