磷酸鞘胺醇调节小鼠单核巨噬细胞炎性细胞因子表达的机制研究

doi:10.3969/j.issn.1006-7795.2015.05.013

首都医科大学学报 ›› 2015, Vol. 36 ›› Issue (5): 729-733.doi: 10.3969/j.issn.1006-7795.2015.05.013

磷酸鞘胺醇调节小鼠单核巨噬细胞炎性细胞因子表达的机制研究

张媛媛, 李伟阳, 杨琳, 李丽英

首都医科大学基础医学院细胞生物学系肝脏保护与再生调节北京市重点实验室, 北京 100069

收稿日期:2015-04-21 出版日期:2015-10-21 发布日期:2015-10-20
基金资助:
国家自然科学基金(81430013), 北京市属高等学校创新团队建设与教师职业发展计划项目(IDHT20150502)。

Regulation of inflammatory cytokine expression of sphingosine 1-phosphate in murine monocyte/macrophage J774A.1

Zhang Yuanyuan, Li Weiyang, Yang Lin, Li Liying

Department of Cell Biology, Municipal Laboratory for Liver Protection and Regulation of Regeneration, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2015-04-21 Online:2015-10-21 Published:2015-10-20
Contact: 李丽英 E-mail:liliying@ccmu.edu.cn
Supported by:
This study was supported by National Natural Science Foundation of China (81430013), The Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges Under Beijing Municipality (IDHT20150502).

摘要/Abstract

摘要： 目的本课题探究了磷酸鞘胺醇(sphingosine 1-phosphate, S1P)对小鼠单核巨噬细胞J774A.1表达炎性细胞因子的调节机制。方法应用蛋白印迹实验检测小鼠单核巨噬细胞J774A.1是否表达S1P受体,进一步应用实时荧光定量聚合酶链反应检测小鼠单核巨噬细胞J774A.1在S1P刺激下是否表达炎性细胞因子,最后应用药理学工具干预S1P受体(S1P receptor, S1PRs)信号系统研究小鼠单核巨噬细胞J774A.1表达炎性细胞因子的调节机制。结果 S1PR₂拮抗剂(JTE-013)、S1PR₃拮抗剂(CAY-10444)可抑制小鼠单核巨噬细胞系J774A.1炎性细胞因子的表达;而S1PR₁拮抗剂(W146)对小鼠单核巨噬细胞炎性细胞因子的表达并无影响。结论磷酸鞘胺醇通过S1PR₂和S1PR₃上调小鼠单核巨噬细胞J774A.1炎性细胞因子的表达。

关键词: 单核巨噬细胞, 磷酸鞘胺醇, 炎性反应因子

Abstract: Objective The aim of study is to explore the role of sphingosine 1-phosphate (S1P) signaling on murine monocyte/macrophage inflammatory cytokine expression. Methods Western blotting was employed to test whether S1P receptors (S1PRs) expression in murine monocyte/macrophages. Then we detected whether S1P-mediated expression of inflammatory cytokines in murine monocyte/macrophage J774A.1 by real-time PCR. We studied regulatory mechanisms of expression of inflammatory cytokines in J774A.1 cells by intervention of S1P/S1PRs signaling with pharmacology tools. Results S1PR₂ antagonist JTE-013, S1PR₃ antagonist CAY-10444 treatment reduced expression of inflammatory cytokines in J774A.1 cells. S1PR₁ antagonist W146 has no effects on the expression of inflammatory cytokines in J774A.1 cells. Conclusion S1P up-regulates expression of inflammatory cytokines in J774A.1 cells mediated by S1PR₂ and S1PR₃.

Key words: monocyte/macrophage, sphingosine 1-phosphate, inflammatory cytokine

中图分类号:

Q291

张媛媛, 李伟阳, 杨琳, 李丽英. 磷酸鞘胺醇调节小鼠单核巨噬细胞炎性细胞因子表达的机制研究[J]. 首都医科大学学报, 2015, 36(5): 729-733.

Zhang Yuanyuan, Li Weiyang, Yang Lin, Li Liying. Regulation of inflammatory cytokine expression of sphingosine 1-phosphate in murine monocyte/macrophage J774A.1[J]. Journal of Capital Medical University, 2015, 36(5): 729-733.

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磷酸鞘胺醇调节小鼠单核巨噬细胞炎性细胞因子表达的机制研究

Regulation of inflammatory cytokine expression of sphingosine 1-phosphate in murine monocyte/macrophage J774A.1

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