低温高压一氧化碳(CO)干燥法对离体兔心的保护作用及机制

doi:10.3969/j.issn.1006-7795.2015.05.017

首都医科大学学报 ›› 2015, Vol. 36 ›› Issue (5): 751-756.doi: 10.3969/j.issn.1006-7795.2015.05.017

低温高压一氧化碳(CO)干燥法对离体兔心的保护作用及机制

郭义龙, 张钟, 周鹏宇, 朱平, 黄帅, 郑少忆

广东省心血管病研究所广东省人民医院(广东省医学科学院)心血管外科, 广州 510080

收稿日期:2015-03-06 出版日期:2015-10-21 发布日期:2015-10-20
基金资助:
中华人民共和国科学技术部国际科技合作项目(S2010GR0755)。

Study on the preservation with carbon oxide and heterotopic transplantation of the isolated rabbit heart

Guo Yilong, Zhang Zhong, Zhou Pengyu, Zhu Ping, Huang Shuai, Zheng Shaoyi

Guangdong Cardiovascular Institute, Cardiac Surgery Department, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China

Received:2015-03-06 Online:2015-10-21 Published:2015-10-20
Contact: 郑少忆 E-mail:shaoyi_zsy@sohu.com
Supported by:
This study was supported by International S&T Cooperation (S2010GR0755).

摘要/Abstract

摘要： 目的探讨低温(4 ℃)高压CO(CO=0.8ATA,O₂=3.2ATA,ATA:1个标准大气压)干燥法对离体兔心的保护作用及其机制。方法取72只新西兰兔作为实验对象,采用数字表法随机分为5组,空白对照组(8只),即刻移植组(16只),普通干燥组(16只),低温高压CO干燥保存组(16只),低温HTK浸泡保存组(16只);分别将后3组离体兔心用单纯干燥、低温高压CO干燥及低温HTK液浸泡等方法进行保存18 h,并将保存后的离体兔心进行兔子腹腔异位移植,测定移植兔心复跳率、心肌酶(CK:肌酸激酶;CK-MB:肌酸激酶同工酶;AST:血清门冬氨酸氨基转移酶;LDH:血清乳酸脱氢酶)、炎性反应因子(TNF-α:肿瘤坏死因子-α;IL-1:白细胞介素-1;IL-17:白细胞介素-17;ICAM-1:细胞间黏附因子-1)及行心肌组织苏木精-伊红染色(HE染色)检查。对各项试验指标进行统计分析,进一步探讨低温高压CO干燥对离体兔心的保护效果及其在抗炎性反应方面的作用及其机制。结果低温高压CO干燥保存法较低温HTK液浸泡保存法而言能有效的降低心肌酶(CK-MB、LDH、CK)的量(P值均<0.05),有效抑制炎性反应因子(IL-1、IL-17、TNF-α)的释放(P值均<0.05),减轻心肌细胞损伤。结论低温高压CO干燥保存法对离体兔心的保存效果优于传统的低温HTK液浸泡法。

关键词: 低温高压CO干燥保存, 心肌保护, 心肌再灌注损伤, 心脏移植

Abstract: Objective Investigating the protective effect and mechanism of hypothermia (4 ℃)and desiccation with CO(CO=0.8ATA,O₂=3.2ATA,1ATA=760 mmHg, 1 mmHg=0.133 kPa)on heart preservation. Methods Seventy-two rabbits were used in this experiment; They were divided into 5 groups randomly. Group Ⅰ(eight of them were collected for examination without transplantation or preservation);Group Ⅱ(sixteen of them were divided into this group and the isolated hearts were underwent abdominal heterotopic transplantation without preservation);Group Ⅲ(sixteen of them were divided into this group and the isolated hearts were preserved with desiccation);Group Ⅳ(sixteen of them were divided into this group and the isolated hearts were preserved with desiccation and CO);Group Ⅴ(sixteen of them were divided into this group and the isolate hearts underwent 18 h preserved with HTK solution); Group Ⅲ-Ⅴ were placed in a refrigerator at 4 ℃; And then, the isolated hearts were underwent abdominal heterotopic transplantation. After the heterotopic transplantation, the re-beat rates were counted; 1 h after transplantation, the blood of receptor and the myocardium of donor were collected to measure the enzyme of function and the inflammatory factors of myocardium; Results After the experiment, most of the enzyme of function (CK-MB: creatine kinase-MB; CK: creatine kinase; LDH: lactic dehydrogenase) and the inflammatory factors of myocardium (TNF-α: tumour necrosis factor-α;IL-1: interleukin-1;IL-17: interleukin-17) in Group Ⅲ were lower than those of Group IV (P<0.05); Conclusion The heart preserved with CO was significantly better than that preserved with traditional method. And this new preservation works by reducing the inflammatory factors and alleviating the myocardia reperfusion injury.

Key words: desiccation and CO, myocardia protection, myocardia reperfusion injury, heart transplant

中图分类号:

R332
R617

郭义龙, 张钟, 周鹏宇, 朱平, 黄帅, 郑少忆. 低温高压一氧化碳(CO)干燥法对离体兔心的保护作用及机制[J]. 首都医科大学学报, 2015, 36(5): 751-756.

Guo Yilong, Zhang Zhong, Zhou Pengyu, Zhu Ping, Huang Shuai, Zheng Shaoyi. Study on the preservation with carbon oxide and heterotopic transplantation of the isolated rabbit heart[J]. Journal of Capital Medical University, 2015, 36(5): 751-756.

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低温高压一氧化碳(CO)干燥法对离体兔心的保护作用及机制

Study on the preservation with carbon oxide and heterotopic transplantation of the isolated rabbit heart

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