陈皮、半夏对动脉粥样硬化小鼠PI3K-Akt通路、SOD、MDA、SA-β-gal水平的影响

doi:10.3969/j.issn.1006-7795.2018.06.003

首都医科大学学报 ›› 2018, Vol. 39 ›› Issue (6): 805-809.doi: 10.3969/j.issn.1006-7795.2018.06.003

陈皮、半夏对动脉粥样硬化小鼠PI3K-Akt通路、SOD、MDA、SA-β-gal水平的影响

孙亚男, 黄小波, 粱伟, 陈文强

首都医科大学宣武医院中医科, 北京 100053

收稿日期:2018-09-20 出版日期:2018-11-21 发布日期:2018-12-19
通讯作者: 陈文强 E-mail:wangxianbo638@163.com
基金资助:
国家自然科学基金（81774234，81473641），北京市医院管理局重点医学专业发展计划［中西医结合传染病（ZYLX201707）］，首都卫生发展科研专项（首发2018-1-2172）。

Effects of citrus and pinellia on the levels of PI3K-Akt pathway, SOD, MDA, SA-sap-gal in atherosclerosis mice

Sun Yanan, Huang Xiaobo, Liang Wei, Chen Wenqiang

Department of Traditional Chinese Medicine, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

Received:2018-09-20 Online:2018-11-21 Published:2018-12-19
Supported by:
This study was supported by National Natural Science Foundation of China (81774234, 81473641), Beijing Hospital Administration Key Medical Professional Development Program[Chinese and Western Medicine Integrated Infectious Diseases (ZYLX201707)], Capital Health Development Research Project (2018-1-2172).

摘要/Abstract

摘要： 目的探讨陈皮、半夏治疗动脉粥样硬化的机制。方法将40只Klotho 敲除小鼠采用数字表法随机分为模型组、治疗组（高、中、低剂量组，每组10只）。对照组C57BL/6小鼠10只。通过HE染色观察小鼠颈动脉病理改变；通过血浆分光光度法检测丙二醛（malonaldehyde，MDA）、超氧化物歧化酶（superoxide dismutase，SOD）、β-半乳糖苷酶（senescence-associated beta-galactosidase，SA-β-gal）；通过免疫组化法观察PI3K与p-Akt表达。结果与模型组比较，陈皮、半夏组动脉内膜呈轻度增生改变；SOD浓度与对照组比较上升，差异有统计学意义（P<0.01），MDA和SA-β-gal则显著降低（P<0.01）；大剂量和中剂量治疗组中PI3K下降水平与模型组比较，差异有统计学意义（P<0.01）；陈皮、半夏各治疗组p-Akt表达均显著低于模型组，差异有统计学意义（P<0.01）。结论陈皮、半夏可能通过PI3K和p-Akt通路而调控SOD、MDA及SA-β-gal水平，达到延缓衰老、治疗动脉粥样硬化的作用。

关键词: 陈皮, 半夏, 衰老, 动脉粥样硬化, PI3K, p-Akt

Abstract: Objective To explore the mechanism of treating atherosclerosis with Citrus and Pinellia. Methods A total of 50 Klotho knockout mice were randomly divided into the normal group, the model group and the treatment group (high, medium and low dose group). The pathological changes of carotid artery were observed with HE staining. The plasma of malondialdehyde(mDA), superoxide dismutase(SOD), senescence-associated beta-galactosidas (SA-β-gal) and the expression of PI3K and p-Akt was examined with immunohistochemistry. Results Compared with the model group, the arterial intima in the Citrus and Pinellia group showed mild hyperplasia. Compared with the control group, the level of SOD increased significantly (P<0.01), while the content of MDA and SA-β-gal decreased significantly (P<0.01). Compared with the model group, the decrease level of PI3K in the high and medium-dose treatment groups was statistically significant (P<0.01), P-Akt expression in the treated group was significantly lower than that in the model group, and the difference was statistically significant (P<0.01). Conclusion Citrus and Pinellia may regulate the levels of SOD, MDA and SA-β-gal through PI3K and p-Akt pathway, to delay senescence and treat atherosclerosis.

Key words: Citrus, Pinellia, aging, atherosclerosis, PI3K, p-Akt

中图分类号:

R285.5

孙亚男, 黄小波, 粱伟, 陈文强. 陈皮、半夏对动脉粥样硬化小鼠PI3K-Akt通路、SOD、MDA、SA-β-gal水平的影响[J]. 首都医科大学学报, 2018, 39(6): 805-809.

Sun Yanan, Huang Xiaobo, Liang Wei, Chen Wenqiang. Effects of citrus and pinellia on the levels of PI3K-Akt pathway, SOD, MDA, SA-sap-gal in atherosclerosis mice[J]. Journal of Capital Medical University, 2018, 39(6): 805-809.

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陈皮、半夏对动脉粥样硬化小鼠PI3K-Akt通路、SOD、MDA、SA-β-gal水平的影响

Effects of citrus and pinellia on the levels of PI3K-Akt pathway, SOD, MDA, SA-sap-gal in atherosclerosis mice

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