关键词: 基因芯片, 人乳头瘤状病毒, 基因亚型

Abstract: Objective To evaluate prevalence of human papillomavirus(HPV) infection and genotypes of the virus in 400 women who underwent health checkup. Methods HPV DNA was determined from the cervical epithelium of 400 women by means of gene chip technique. The overall HPV prevalence and age distribution in the 400 women were analyzed. Results HPV DNA was detected in 17.0% of all the women(68 of 400 cases), The high risk HPV was detected in 61.8% of the HPV-positive women(42 of 68 cases) and HPV 16 was the most common type. The low risk HPV was detected in 22.1% of the HPV-positive women(15 of 68 cases), HPV43 and HPV 11 were the most common types. The combined high and low risk HPV was detected in 16.1% of the HPV-positive women(11 of 68 cases). The highest HPV prevalence was found in women at the ages of 20~34 years(18.2%) compared with the women aged ≤19 years(15.4%) and aged ≥ 35 years(12.7%) , but there were no significant differences among the age groups. Conclusion Gene chip technique detects a high prevalence of HPV in the women who underwent health checkup.

Key words: gene chip technique, human papillomavirus, genotype