特异性小干扰RNA抑制诱骗受体3在结肠癌SW480细胞表达的研究

doi:10.3969/j.issn.1006-7795.2012.06.019

首都医科大学学报 ›› 2012, Vol. 33 ›› Issue (6): 798-802.doi: 10.3969/j.issn.1006-7795.2012.06.019

特异性小干扰RNA抑制诱骗受体3在结肠癌SW480细胞表达的研究

何平¹, 伍冀湘¹, 钟晨熙¹, 戴洁², 梁杰雄¹, 李洋¹, 许英晨¹, 于玮²

1. 首都医科大学附属北京安贞医院普外科, 北京 100029;2. 首都医科大学基础医学院病理学系, 北京 100069

收稿日期:2012-05-14 修回日期:1900-01-01 出版日期:2012-12-21 发布日期:2012-12-21
通讯作者: 伍冀湘

Down-regulation of DcR3 expression by siRNA in SW480 cells

HE Ping¹, WU Jixiang¹, ZHONG Chenxi¹, DAI Jie², LIANG Jiexiong¹, LI Yang¹, XU Yingcheng¹, YU Wei²

1. Department of General Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China;2. Department of Pathology, School of Basic Medical Science, Capital Medical University, Beijing 100069, China

Received:2012-05-14 Revised:1900-01-01 Online:2012-12-21 Published:2012-12-21

摘要/Abstract

摘要： 目的研究特异性小干扰RNA(small interfering RNA,siRNA)对诱骗受体3(decoy receptor 3,DcR3)基因在人结肠癌SW480细胞系中的抑制作用。方法设计特异性的DcR3 siRNA序列;构建DcR3的pSUPER表达质粒并鉴定,鉴定成功后用于转染SW480细胞。以RT-PCR的方法检测细胞转染后DcR3 mRNA的表达;以Western blotting的方法检测DcR3蛋白的表达情况,并作统计学处理。结果与对照组相比,本实验设计两段siRNA对SW480细胞中DcR3 mRNA及蛋白表达的抑制作用明显,实验细胞DcR3 mRNA及蛋白表达显著下降(P<0.01)。结论针对DcR3的siRNA质粒转染真核细胞后可抑制其mRNA及蛋白表达,为进一步探讨DcR3的功能及封闭DcR3基因表达而治疗肿瘤奠定了实验基础。

关键词: 诱骗受体3, 小干扰RNA, 结肠癌细胞

Abstract: Objective To investigate the inhibitory effect of small interfering RNA(siRNA) on the expression of decoy receptor 3(DcR3) in human colon carcinoma cell line SW480. Methods Two siRNA plasmid expression vectors targeting DcR3 were constructed and transfected into SW480 cells with Lipofectamine 2000. The down regulation of DcR3 expressions were detected by RT-PCR and Western blotting methods, following statistical processing. Results Compared with the control group, the sequence-specific siRNAs efficiently and specifically down-regulated the expression of DcR3 at both mRNA and protein levels. The expression inhibition of mRNA and protein of DcR3 significantly decreased(P<0.01). Conclusion The siRNA plasmid expression vector against DcR3 can inhibit the expression of DcR3 in SW480 cells efficiently and specifically. siRNA against DcR3 may be of potential value in gene therapy of cancer.

Key words: decoy receptor 3, small interfering RNA, colon carcinoma cell

中图分类号:

何平;伍冀湘;钟晨熙;戴洁;梁杰雄;李洋;许英晨;于玮. 特异性小干扰RNA抑制诱骗受体3在结肠癌SW480细胞表达的研究[J]. 首都医科大学学报, 2012, 33(6): 798-802.

HE Ping;WU Jixiang;ZHONG Chenxi;DAI Jie;LIANG Jiexiong;LI Yang;XU Yingcheng;YU Wei. Down-regulation of DcR3 expression by siRNA in SW480 cells[J]. Journal of Capital Medical University, 2012, 33(6): 798-802.

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特异性小干扰RNA抑制诱骗受体3在结肠癌SW480细胞表达的研究

Down-regulation of DcR3 expression by siRNA in SW480 cells

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