糖基化终末产物损伤冠状动脉平滑肌细胞Kv通道

doi:10.3969/j.issn.1006-7795.2016.06.002

首都医科大学学报 ›› 2016, Vol. 37 ›› Issue (6): 725-730.doi: 10.3969/j.issn.1006-7795.2016.06.002

• 心血管疾病的病理生理机制 • 上一篇下一篇

糖基化终末产物损伤冠状动脉平滑肌细胞K_v通道

苏文¹, 李虹伟¹, 陈晖¹, 刘慧荣², 黄海霞², 李卫萍¹

1. 首都医科大学附属北京友谊医院心血管中心, 北京 100050;
2. 首都医科大学基础医学院生理学与病理生理学系, 北京 100069

收稿日期:2016-10-03 出版日期:2016-12-21 发布日期:2016-12-16
通讯作者: 李卫萍 E-mail:xueer09@163.com
基金资助:
国家自然科学基金（30971240），北京市自然科学基金（7122053），北京市卫生系统高层次卫生技术人才资助项目（2013-3-060）

Advanced glycation end products impair voltage-gated K⁺ channels in coronary vascular smooth muscle cells

Su Wen¹, Li Hongwei¹, Chen Hui¹, Liu Huirong², Huang Haixia², Li Weiping¹

1. Department of Cardiology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China;
2. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2016-10-03 Online:2016-12-21 Published:2016-12-16
Supported by:
This study was supported by National Natural Science Foundation of China(30971240), Natural Science Foundation of Beijing (7122053),High-level Technical Talents Foundation in Beijing Health System (2013-3-060).

摘要/Abstract

摘要： 目的研究糖基化终末产物（advanced glycation end products，AGEs）对大鼠冠状动脉平滑肌细胞电压门控性钾离子（voltage-gated K⁺，K_v）通道电流的影响。方法分离大鼠冠状动脉平滑肌细胞，分为空白对照组、糖基化牛血清白蛋白（AGE-bull serum albumin，AGE-BSA）组、AGE-BSA+抗AGE受体抗体（anti-receptor of AGEs immunoglobulin G，anti-RAGE IgG）组进行干预。采用膜片钳技术检测各组细胞K_v通道电流，采用Western blotting、实时荧光定量PCR方法检测各组细胞K_v1.2和K_v1.5通道蛋白及mRNA的表达。结果 AGE-BSA直接干预冠状动脉平滑肌细胞明显抑制了平滑肌细胞的K_v电流达32.7%，并使K_v1.2和K_v1.5通道蛋白及mRNA的表达明显下调。而给予anti-RAGE IgG预处理30 min后再加入AGE-BSA刺激，平滑肌细胞的Kv电流密度及Kv1.2和Kv1.5通道蛋白及mRNA的表达与空白对照组相比，差异无统计学意义。结论 AGEs通过结合RAGE损伤冠状动脉平滑肌细胞K_v通道。

关键词: 糖基化终末产物, 电压门控性钾离子通道, 冠状动脉平滑肌细胞, 糖基化终末产物受体

Abstract: Objective To investigate the role of advanced glycation end products (AGEs) in impairment of voltage-gated K⁺(K_v) channels in rat coronary vascular smooth muscle cells (VSMCs). Methods We isolated rat coronary VSMCs. The cells were incubated either in control medium, or medium with 100 μg/mL AGE-bovine serum albumin (AGE-BSA), or medium with 100 μg/mL AGE-BSA plus 100 μg/mL anti-receptor of AGEs immunoglobulin G (anti-RAGE IgG). Patch-clamp recording was used to assess the K_v currents. Western blotting and quantitative real-time PCR techniques were used to assess protein and mRNA expression of K_v 1.2 and K_v1.5. Results Incubation of VSMCs with AGE-BSA reduced K_v current density by 32.7% and decreased both protein and mRNA expression of K_v1.2 and K_v1.5 channels, whereas blocking AGE-BSA interacting with their receptors by preincubation with anti-RAGE IgG for 30 minutes prevented AGE-BSA-induced impairment of K_v channels.Conclusion AGEs impair K_v channels in coronary VSMCs by interacting with RAGE.

Key words: advanced glycation end products, voltage-gated K⁺ channels, coronary vascular smooth muscle cells, receptor of advanced glycation end products

中图分类号:

苏文, 李虹伟, 陈晖, 刘慧荣, 黄海霞, 李卫萍. 糖基化终末产物损伤冠状动脉平滑肌细胞K_v通道[J]. 首都医科大学学报, 2016, 37(6): 725-730.

Su Wen, Li Hongwei, Chen Hui, Liu Huirong, Huang Haixia, Li Weiping. Advanced glycation end products impair voltage-gated K⁺ channels in coronary vascular smooth muscle cells[J]. Journal of Capital Medical University, 2016, 37(6): 725-730.

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糖基化终末产物损伤冠状动脉平滑肌细胞K_v通道

Advanced glycation end products impair voltage-gated K⁺ channels in coronary vascular smooth muscle cells

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糖基化终末产物损伤冠状动脉平滑肌细胞Kv通道

Advanced glycation end products impair voltage-gated K+ channels in coronary vascular smooth muscle cells

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糖基化终末产物损伤冠状动脉平滑肌细胞K_v通道

Advanced glycation end products impair voltage-gated K⁺ channels in coronary vascular smooth muscle cells