miR-378a-5p下调人肝脏星形细胞系LX-2中鞘胺醇激酶1的表达

doi:10.3969/j.issn.1006-7795.2019.01.017

首都医科大学学报 ›› 2019, Vol. 40 ›› Issue (1): 94-100.doi: 10.3969/j.issn.1006-7795.2019.01.017

miR-378a-5p下调人肝脏星形细胞系LX-2中鞘胺醇激酶1的表达

祁长波, 常娜, 杨琳, 李丽英

首都医科大学细胞生物学系‘肝脏保护与再生调节’北京市重点实验室, 北京 100069

收稿日期:2018-11-01 出版日期:2019-01-21 发布日期:2019-01-23
通讯作者: 李丽英 E-mail:liliying@ccmu.edu.cn
基金资助:
国家自然科学基金（81770603），北京市自然科学基金（7172019）。

miR-378a-5p down-regulates SphK1 expression in LX-2

Qi Changbo, Chang Na, Yang Lin, Li Liying

Department of Cell Biology, Municipal Laboratory for Liver Protection and Regulation of Regeneration, Capital Medical University, Beijing 100069, China

Received:2018-11-01 Online:2019-01-21 Published:2019-01-23
Supported by:
This study was supported by National Natural Science Foundation of China(81170603), Natural Science Foundation of Beijing (7172019).

摘要/Abstract

摘要： 目的预测、筛选并探究是否存在特定的microRNA能够影响人肝脏星形细胞LX-2中鞘胺醇激酶1（sphingosine kinase 1，SphK1）mRNA的表达。方法采用生物信息学数据库预测microRNA，采用实时荧光定量聚合酶链式反应（real-time quantitative polymerase chain reaction，RT-qPCR）检测SphK1 mRNA的表达。结果预测筛选出的miR-378a-5p、miR-92a-2-5p和miR-708-5p中，只有miR-378a-5p能够抑制LX-2中由转化生长因子β1（transforming growth factor-β1，TGF-β1）介导的SphK1 mRNA表达上调这一过程。而在LX-2中转染miR-378a-5p的抑制剂阻断其作用后，SphK1 mRNA的表达上调。结论 miR-378a-5p参与LX-2中SphK1 mRNA表达的调控，并且能够抑制由TGF-β1介导的SphK1 mRNA表达上调。

关键词: miR-378a-5p, 鞘胺醇激酶1, 转化生长因子β1

Abstract: Objective To investigate whether microRNA could regulate the expression of sphingosine kinase 1 (SphK1) mRNA in LX-2 cells. Methods Prediction of microRNAs was performed on bioinformatics sites. Real-time quantitative polymerase chain reaction (RT-qPCR) was applied to detect the expression of SphK1 mRNA in LX-2 cells. Results Only miR-378a-5p, not miR-92a-2-5p or miR-708-5p, could suppress the increase of SphK1 mRNA expression caused by transforming growth factor-β1 (TGF-β1) in LX-2. And the expression of SphK1 mRNA was up-regulated when the inhibitor of miR-378a-5p was applied in LX-2 cells. Conclusion miR-378a-5p took part in the regulation of SphK1 mRNA expression in LX-2, and it could down-regulate the increase of SphK1 mRNA expression caused by TGF-β1.

Key words: miR-378a-5p, sphingosine kinase 1 (SphK1), transforming growth factor-β1

中图分类号:

R329.2

祁长波, 常娜, 杨琳, 李丽英. miR-378a-5p下调人肝脏星形细胞系LX-2中鞘胺醇激酶1的表达[J]. 首都医科大学学报, 2019, 40(1): 94-100.

Qi Changbo, Chang Na, Yang Lin, Li Liying. miR-378a-5p down-regulates SphK1 expression in LX-2[J]. Journal of Capital Medical University, 2019, 40(1): 94-100.

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miR-378a-5p下调人肝脏星形细胞系LX-2中鞘胺醇激酶1的表达

miR-378a-5p down-regulates SphK1 expression in LX-2

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