首都医科大学学报 ›› 2026, Vol. 47 ›› Issue (4): 658-664.doi: 10.3969/j.issn.1006-7795.2026.04.004

• 更年期妇科内分泌与生育力保护 • 上一篇    下一篇

雌激素替代治疗对裸鼠人卵巢组织异种移植后内分泌功能恢复的影响

蒋玲玲1,阮祥燕1,2*,杜娟1,柳岸鸣1,程姣姣1,谷牧青1,李扬璐1,张明珍1,王泽铖1,李妍秋1,Alfred O. Mueck1,2
  

  1. 1. 首都医科大学附属北京妇产医院/北京妇幼保健院内分泌科, 北京 100026; 2. 德国图宾根大学妇产医院妇女健康部与妇女健康研究中心,图宾根 D-72076
  • 收稿日期:2026-04-09 修回日期:2026-04-21 出版日期:2026-08-21 发布日期:2026-07-26
  • 通讯作者: 阮祥燕 E-mail:ruanxiangyan@ccmu.edu.cn
  • 基金资助:
    北京市医院管理局“登峰”人才培养计划项目（DFL20181401），北京市卫生健康委员会北京市研究型病房示范建设项目：生育力保护及卵巢组织冻存移植技术的临床推广研究（BCRW202109），国家外国专家个人类项目生育力保护与妇科内分泌疑难病研究会诊中心的建立项目（S20240236)，国家外国专家个人类项目全国女性卵巢保护与抗衰促进工程项目建设（S20240230）。 

Effects of estrogen replacement therapy on the recovery of endocrine function after xenotransplantation of human ovarian tissue in nude mice

Jiang Lingling1, Ruan Xiangyan1,2*, Du Juan1, Liu Anming1, Cheng Jiaojiao1, Gu Muqing1, Li Yanglu1, Zhang Mingzhen1, Wang Zecheng1, Li Yanqiu1 , Alfred O.Mueck1,2   

  1. 1.Department of Gynecological Endocrinology，Beijing Obstetrics and Gynecology Hospital, Capital Medical University. Beijing Maternal and Child Health Care Hospital, Beijing 100026, China; 2.Department of Women's Health,  Research Centre for Women's Health and University Women's Hospital of Tuebingen， University Hospitals of Tuebingen, Tuebingen D-72076, Germany
  • Received:2026-04-09 Revised:2026-04-21 Online:2026-08-21 Published:2026-07-26
  • Supported by:
    This study was supported by Beijing Municipal Administration of Hospitals “Dengfeng” Talent Training Program (DFL20181401)， Beijing Municipal Health Commission Demonstration Construction Project of Research-Oriented Wards: Clinical Promotion Research on Fertility Preservation and Ovarian Tissue Cryopreservation and Transplantation Technology (BCRW202109)， National Foreign Experts Individual Project, Establishment of Fertility Preservation and Consultation Center for Gynecological Endocrine Difficult Diseases (S20240236), National Foreign Experts Individual Project, Construction of National Female Ovarian Preservation and Anti-aging Promotion Project (S20240230).

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摘要： 目的  评价不同雌激素干预策略对裸鼠人卵巢组织异种移植后卵巢内分泌功能恢复的影响。方法  选取6~8周龄无特定病原体（specific pathogen free，SPF）级雌性BALB/c裸鼠，双侧卵巢切除建立去势模型。除对照组外，各组均行人卵巢皮质组织肾被膜下移植。雌激素替代治疗（estrogen replacement therapy，ERT）采用皮下植入17β-雌二醇缓释片（0.72 mg/片）。按干预方式分为Pre-ERT组（仅在移植前7 d进行干预）、C-ERT组（从移植前7 d持续至终点）、No-ERT组及control组。卵巢组织移植后7 d和21 d采集裸鼠血清，采用酶联免疫吸附实验（enzyme linked immunosorbent assay, ELISA）法检测促卵泡生成素（follicle-stimulating hormone，FSH）、雌二醇（estradiol，E2）及抗米勒管激素（anti-Müllerian hormone，AMH）浓度。结果  移植后第7天，Pre-ERT组与C-ERT组E2及AMH浓度均高于No-ERT组，且各移植组均高于control组（P<0.05），FSH浓度低于No-ERT组（P<0.05），control组高于各移植组（P<0.05）。第21天，Pre-ERT组与C-ERT组E2及AMH浓度均高于No-ERT组（P<0.05），FSH组间差异消失，但control组仍高于各移植组（P<0.05）。结论  ERT有助于促进冻存卵巢组织移植后的内分泌功能恢复，其中持续性雌激素治疗较移植前预处理在加速功能恢复方面效果更为显著。

关键词: 卵巢组织冻存与移植, 雌激素替代治疗, 抗米勒管激素, 裸鼠, 异种移植, 卵巢内分泌功能

Abstract: Objective  To investigate the effects of estrogen replacement therapy (ERT) on the recovery of ovarian endocrine function after human ovarian tissue xenotransplantation in nude mice. Methods  Female BALB/c nude mice aged 6-8 weeks were subjected to bilateral ovariectomy to establish a castrated model. Except for the control group, all mice underwent xenotransplantation of human ovarian cortical tissue under the renal capsule. ERT was administered via subcutaneous implantation of 17β-estradiol slow-release pellets (0.72 mg/pellet). Animals were divided into four groups according to the timing of estrogen intervention: pre-transplant estrogen pretreatment (Pre-ERT), continuous estrogen treatment (C-ERT), no estrogen treatment (No-ERT), and control group. Serum samples were collected at days 7 and 21 after transplantation, and levels of follicle-stimulating hormone (FSH), estradiol (E2), and anti-Müllerian hormone (AMH) were measured using ELISA. Results  At day 7, serum FSH levels in the control group were significantly higher than those in all transplantation groups, while the Pre-ERT group showed lower FSH levels than the No-ERT group. The C-ERT group exhibited the highest E2 levels, with all transplantation groups showing elevated E2 levels compared to the control group. AMH levels in the C-ERT group were higher than those in the No-ERT group, with all transplantation groups showed higher AMH levels than the control group (all P < 0.05). At day 21, FSH levels in the control group remained higher than those in all transplantation groups. Both Pre-ERT and C-ERT groups showed higher E2 levels than the No-ERT and control groups. AMH levels in the C-ERT group were higher than those in the No-ERT group, and all transplantation groups showing higher AMH levels relative to the control group (all P < 0.05). Conclusion  ERT improves the recovery of endocrine function after ovarian tissue xenotransplantation. Continuous estrogen treatment appears to be more effective than pre-transplant pretreatment in maintaining E2 and AMH levels, possibly by improving the local microenvironment and supporting graft function.

Key words: ovarian tissue cryopreservation and transplantation, estrogen replacement therapy, anti-Müllerian hormone, nude mice, xenotransplantation, ovarian endocrine function

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