首都医科大学学报 ›› 1984, Vol. 5 ›› Issue (2): 134-142.

• 论著 • 上一篇    下一篇

北京地区汉族人白细胞抗原系统(HLA)的研究Ⅱ淋巴细胞毒抗血清特异性分析与鉴定

北京市白细胞分型协作组   

  1. 北京市白细胞分型协作组
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1984-04-15 发布日期:1984-04-15

Study on the Leucocyte Antigen System (HLA) of the Han Nationality Population in Beijing Area:Ⅱ.The Analysis and the Identification of Specificities of Lymphocytotoxicity Antisera

  • Received:1900-01-01 Revised:1900-01-01 Online:1984-04-15 Published:1984-04-15

摘要: 本研究应用微量淋巴细胞毒实验筛选出来的261支强阳性抗体血清,在三个棋盘实验中,同83支包含44个特异性的国际标准血清,39个已知型别抗原指定区及北京地区汉族人随机人群(n=92)的已知型别“标准细胞”进行关联分析。通过聚类分析、包含分析、补充聚类包含分析等多条计算途径的分析,得到检定出型别的抗血清162支,包含有39个特异性。其后,我们将这些捡出型别血清,先后分别又在两个棋盘中(应用标准细胞)再次进行了鉴定,挑选出可作为分型试剂使用的血清82支,计包含有25种特异性,其中除掉只有单支血清的特异性之外,可组合成用以鉴定18种抗原特异性的配组血清。

Abstract: Establishing typing reagents of Chinese HLA,and standard HLA antisera,for filling some blanks in the medical scientific research is one of the problems of utmost importance.For this reasons a co-rrelative analysis was made on 261 strong by positive antisera which were screening out by using the micro lymphocyte cytotoxic test, with 83 international standard an-tisera including 44 specificities, and also with 39 known typing an-tigens assignment regions.Through analyses basing upon multiple cal-culation methods (clustering ana-lysis,includiug analysis,supple-mented clustering and including analysis,etc.)162 antisera of known types including 39 specifi-cities were obtained.Later on,the-se antisera of known types were identified again in two chessboa-rds.We selected 82 antisera inclu-ding 25 specificities which can be used as typing reagents.Except for those specificities with only one antiserum,these antisera had 18 compatibilities in order to deter-mine the antigens′ specificities.