首都医科大学学报 ›› 1984, Vol. 5 ›› Issue (3): 191-195.

• 论著 • 上一篇    下一篇

绿脓杆菌凝集素(Lectin)的研究:Ⅰ凝集素的产生与对上皮细胞的粘附和蛋白酶分泌的关系

曹祖华1, 黄恕2, 孙嘉艳1, 程松高1   

  1. 1. 北京第二医学院微生物教研室;2. 张家口医学院 进修教师
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1984-07-15 发布日期:1984-07-15

Studies on Lectin of Pseudomonas Aeruginose

Cao Zuhua1   

  • Received:1900-01-01 Revised:1900-01-01 Online:1984-07-15 Published:1984-07-15

摘要: 我们对23株绿脓杆菌(PA)菌株的凝集素产生能力与对上皮细胞的粘附以及胞外蛋白酶活性的关系进行了研究,结果发现:1)12株产生高滴度凝集素(1∶128—1∶512),1株产生低滴度凝集素(1∶16),其余9株不产生凝集素。2)产生凝集素的菌株比不产生或产生低滴度的菌株对上皮细胞有更强的粘附力。3)产生凝集素菌株,胞外蛋白酶活性也高,不产生凝集素菌株,胞外蛋白酶活性很低或捡不出。4)半乳糖能抑制凝集素的血凝活性并能部分抑制细菌的粘附作用。

Abstract: The relationship between the pro-duction of Lectin and adherence to epithelial cells together with extracellutar protease activity in 23 strains of P.aeruginose (PA) was studien The findings are as follows: 1.12 strains were high-Lectin pro-ducers (1:128-1:512),one strain was the low-Lectin producer (1:16), the rcmaning 9 strains were non-Lectin producers 2.Adherent ability of the former was stronger than that of the lat-ter two. 3.The proteaseactivity produced in the Lectin-producer was signi-ficantly higher than that produced in the non-Lectin producer 4.The hemagglutinating activity of Lectin was inhibited by galac-tose and the bacterial adherence was in part inhibited.