绿脓杆菌凝集素的研究——Ⅲ.半乳糖专一性绿脓杆菌凝集素的特性

首都医科大学学报 ›› 1985, Vol. 6 ›› Issue (2): 120-124.

绿脓杆菌凝集素的研究——Ⅲ.半乳糖专一性绿脓杆菌凝集素的特性

曹祖华, 程松高

北京第二医学院微生物学教研室

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1985-04-15 发布日期:1985-04-15

Studies on Pseudomonas Aeruginosa Lectin Ⅲ: Characterization of Galactose--specific Lectin from Pseudomonas Aeruginosa

Cao Zuhua

Received:1900-01-01 Revised:1900-01-01 Online:1985-04-15 Published:1985-04-15

摘要/Abstract

摘要： 半乳糖专一的绿脓杆菌凝集素(PA-Lectin)能凝集包括大鼠、家兔、小鼠、羊、猪等8种动物的红细胞,除鸡红细胞外,用木瓜蛋白酶处理的红细胞的凝集能力比未处理红细胞要强。半乳糖,N—乙酰半乳糖胺,乳糖、密二糖、棉子糖、木糖和Na-EDTA能抑制PA—Lectin的血凝作用,但葡萄糖和N-乙酰葡葡糖胺无抑制作用。用分光光度分析法测定半乳糖与PA-Lectin的结合,其结合常数为6×10³L/Mol。此外,凝胶过滤分析表明,PA-Lectin的单体易于聚合。

Abstract: Galactose-sepecific lectin from pseudomonas aeruginosa could agglutinate the erythrocytes of various animals (including rat, rabbit, mouse, sheep and pig) and the ability to agglutinate the erythrocytes treated with papain was stronger than those untreated except erythrocyte of chicken. The hemagglutination activity of lectin was inhibited by galactose, N-acetyl-galactosamine, lactose, raffinose, melibiose, xylosa and Na-EDTA, but not by glucose and N-acetylglu-cosamine. Binding of galactose to the lectin was assayed spectro-photometrically and the association constant for galactose was 6×10³L/ Mol. In addition, gel-filtration analysis revealed that the monomer of the lectin exhibited a tendency of aggregation.

曹祖华;程松高. 绿脓杆菌凝集素的研究——Ⅲ.半乳糖专一性绿脓杆菌凝集素的特性[J]. 首都医科大学学报, 1985, 6(2): 120-124.

Cao Zuhua. Studies on Pseudomonas Aeruginosa Lectin Ⅲ: Characterization of Galactose--specific Lectin from Pseudomonas Aeruginosa[J]. Journal of Capital Medical University, 1985, 6(2): 120-124.