应用固相抗HBs双抗体ELISA技术检测慢性HB患者血清中HBsAg特异性免疫复合物

首都医科大学学报 ›› 1985, Vol. 6 ›› Issue (2): 95-100.

应用固相抗HBs双抗体ELISA技术检测慢性HB患者血清中HBsAg特异性免疫复合物

温蒞, 韩钦, 曹祖华, 陈仁

北京第二医学院微生物学教研室

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1985-04-15 发布日期:1985-04-15

The Detection of HBsAg--specific CIC in Patients with Chronic HB by Double Antibody ELISA Using Anti--HBs Attached on Solid Phase

Wen Li

Received:1900-01-01 Revised:1900-01-01 Online:1985-04-15 Published:1985-04-15

摘要/Abstract

摘要： 介绍一种应用固相抗HBs-BLISA技术分类检测IgG和IgM类抗体形成的HBsAg特异性CIC的方法。本方法最适检出抗原稍过量的HBsAg-CIC。对不同类型慢性HB患者检测的结果表明,HBAC、CAH较CPH的HBsAg-CIC检出率高,且IgM类抗体形成的HBsAg—CIC与病型区别和活动性肝损害的关系更为密切。

Abstract: An assay for the specific detection of HBsAg-CIC composed of different immunoglobulins (IgG and IgM) by ELISA using anti-HBs attached on solid phase was reported in this paper. thismethod was most suitable for the detection of HBsAg-CIC with slightly exce-ssive antigen. The results of the detection in different types of patients with chronic HB showed that the incidence of HBsAg-CIC in HBAC and CAH was remarkably higher than that in CPH, in ad-dition, BHsAg/IgM was more close-ly related with the difference of disease types and with the activity of liver damage.

温蒞;韩钦;曹祖华;陈仁. 应用固相抗HBs双抗体ELISA技术检测慢性HB患者血清中HBsAg特异性免疫复合物[J]. 首都医科大学学报, 1985, 6(2): 95-100.

Wen Li. The Detection of HBsAg--specific CIC in Patients with Chronic HB by Double Antibody ELISA Using Anti--HBs Attached on Solid Phase[J]. Journal of Capital Medical University, 1985, 6(2): 95-100.