曼氏血吸虫成虫确定抗原的研究

首都医科大学学报 ›› 1987, Vol. 8 ›› Issue (4): 264-267.

曼氏血吸虫成虫确定抗原的研究

石梦辉

首都医学院寄生虫学教研室

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1987-10-15 发布日期:1987-10-15

Studies on Defined Antigen of Adult Worm of Schistosoma mansoni

Shi Menghui

Department of Parasitology

Received:1900-01-01 Revised:1900-01-01 Online:1987-10-15 Published:1987-10-15

摘要/Abstract

摘要： 用经放射线照射的曼氏血吸虫(Schistosoma mansoni)尾蚴感染的小鼠血清,对转移到硝化纤维素纸(nitrocellulose paper)上的曼氏血吸虫成虫可溶性抗原进行免疫检测,发现该血清明显识别成虫21KDa可溶性抗原。将与此抗原结合的抗体洗脱,并用洗脱的抗体对曼氏血吸虫生活史各期的可溶性抗原进行酶联免疫吸附试验(EDISA),结果均为阳性。从而表明,曼氏血吸虫成虫21KDa可溶性抗原是曼氏血吸虫成虫的确定抗原(defined antigen)。此抗原不是期待异的,而是曼氏血吸虫生活史中各个时期的共同抗原。本研究结果也说明,曼氏血吸虫成虫21KDa可溶性抗原为曼氏血吸虫成虫的表面抗原。

关键词: 曼氏血吸虫, 转移电泳, 确定抗原

Abstract: Vaccinated serum getting from mouse which vaccinated with irradiated cercariae of S.mansoni.After western blot,using the vaccinated mouse serum to do immunodetection of soluble antigens of adult worm of S.mansoni on nitroeellulose paper.The result appeared that vaccinated mouse serum recognized 21 KDa solubleantigen of adult worm of S.mansoni obviously.The antibody combined with 21 KDa soluble antigen of adult worm on nitrocellulose paper was eluted and using that antibody to do Enzyme-linked immunosorbent assay(ELISA)With soluble antigens of each stage in life history of S.mansoni,all of the results were positive.We conclude that 21 KDa soluble antigen of adult worm of S.mansoni is a defined antigen.This antigen is not stage specific,it is a common antigen of each stage in life history of this parasite.The results indicate that 21 KDa soluble antigen of adult worm of S.mansoni is a surface antigen.

Key words: Schistosoma mansoni, western blot, defined antigen

石梦辉. 曼氏血吸虫成虫确定抗原的研究[J]. 首都医科大学学报, 1987, 8(4): 264-267.

Shi Menghui. Studies on Defined Antigen of Adult Worm of Schistosoma mansoni[J]. Journal of Capital Medical University, 1987, 8(4): 264-267.