应用Feulgen染色及显微分光光度计探讨乙胺嘧啶对约氏疟原虫卵囊的作用机理

首都医科大学学报 ›› 1987, Vol. 8 ›› Issue (4): 280-282.

应用Feulgen染色及显微分光光度计探讨乙胺嘧啶对约氏疟原虫卵囊的作用机理

诸欣平, 孔德芳, 王瑞芳

首都医学院寄生虫学教研室

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1987-10-15 发布日期:1987-10-15

The Effect of Pyrimethamine on the Oocysts of Plasmodium yoelii yoelii

Zhu Xinping

Department of Parasitology

Received:1900-01-01 Revised:1900-01-01 Online:1987-10-15 Published:1987-10-15

摘要/Abstract

摘要： 本实验应用Feulgen组化染色研究乙胺嘧啶作用后约氏疟原虫卵囊内核酸含量的变化,并在显微分光光度计下进行DNA含量测定。结果显示,对照组早、中期卵囊内含有丰富的DNA颗粒,晚期卵囊内形成大量成熟的子孢子。而用药组卵囊发育迟缓,胞浆内来见清晰的DNA团块,仅见色素堆积,部分卵囊形成黑孢子。用药组卵囊的DNA平约含量亦明显低于对照组。初步说明,乙胺嘧啶可抑制疟原虫卵囊的DNA合成,从而阻止了蚊体内疟原虫的继续发育成熟.

关键词: Feulgen染色, 乙胺嘧啶, 约氏疟原虫, 卵囊

Abstract: Feulgen stain and microspectrophotometer were used for the study of the effect of pyrimethamine on the DNAamount of oocysts of Plasmodium yoelii yoelii.The results showed that there were many DNA particles in the early stage of normal oocysts and a lot of mature sporozoits in their late stage.After pyrimethamine was used,the development of oocysts was retarded and some of them formed“black spore”.The amount of DNA in drug-affected oocysts was much lower than the control group.It was indicated that pyrimethamine interfered with DNA synthesis in oocysts,which in turn inhibited the growth of malaria parasites in mosquitoes.

Key words: Feulgen stain, Pyrimethamine, Plasmodium yoelii yoelii, oocyst

诸欣平;孔德芳;王瑞芳. 应用Feulgen染色及显微分光光度计探讨乙胺嘧啶对约氏疟原虫卵囊的作用机理[J]. 首都医科大学学报, 1987, 8(4): 280-282.

Zhu Xinping. The Effect of Pyrimethamine on the Oocysts of Plasmodium yoelii yoelii[J]. Journal of Capital Medical University, 1987, 8(4): 280-282.

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