猪蛔虫与人蛔虫体壁蛋白组分可溶性抗原及同功酶的电泳分析

首都医科大学学报 ›› 1988, Vol. 9 ›› Issue (2): 109-113.

猪蛔虫与人蛔虫体壁蛋白组分可溶性抗原及同功酶的电泳分析

曹伟, 姜洪杰, 陈佩惠, 杨连雪

首都医学院寄生虫学教研室

收稿日期:1987-08-01 修回日期:1900-01-01 出版日期:1988-04-15 发布日期:1988-04-15

Electrop Horetic Analysis on Protein Components, Soluble Antigenic Preparation and Isoenzyme of Body Wall Of Ascaris Suum and Ascaris Lumpricoids

Cao wei

Department of Parasitology, Capital Institute of Medicine

Received:1987-08-01 Revised:1900-01-01 Online:1988-04-15 Published:1988-04-15

摘要/Abstract

摘要： 报告猪蛔虫与人蛔虫体壁蛋白组分,可溶性抗原及乳酸脱氢酶和脂酶同功酶的电泳分析结果。猪蛔虫体壁有3条(PAGE)、18条(SDS-PAGE)和10条(IEF)考马斯亮蓝染色带;人蛔虫体壁相应有11、15、8条染色带,两者的等电点范围为pH3.5～6.4。猪蛔虫体壁至少含9种抗原;人蛔虫体壁至少含7种相关抗原,两种蛔虫体壁脂酶电泳结果表明在酶带的数目及相对迁移率方面均有差异,而两种蛔虫乳酸脱氢酶的电泳结果则无明显差异。

关键词: 猪蛔虫, 人蚵虫, 蛋白组分, 可溶性抗原, 乳酸脱氢酶, 脂酶

Abstract: This paper reports the electrophoretic patterns of the protein compo-nents, soluble body wall antigen (SBWA), isoenzyme of Esterase (EST) and Lactate dehydrogenase (LDH) of the body wall of Ascaris suum, Asearis Lumbricoids. In the somatic complex of A. suum, there were 13 bands in PAGE; 18 bands in SDS-PAGE; 10 bands in IEF stained with coomassie brilliant blue, whereas there Were 11; 15; 8 corresponding abnds in the somatic complex of A. lumbricoids. The body wall of A. suum& A. lumbricoids had isoelectric points of pH values ranging from 3.5~6.4. In IE study, at least 9 precipitate bands of anti-A. suum body wall rabbit serum (aAsBW-RS) against A.1-SBWA Were found, whereas 7 bands of aAsBW-RS against A.1-SBWA were found. The isoenzyme patterns of EST in PAGE and IEF ahowed that there were differences in the number of isoenzymeband and the Rf between A. suum & A. lumbricoids, but no di-fference in the LDH patterns in PAGE Was observed between them.

Key words: Ascaris suun Ascaris lunbricoids, protein components, soluble antigen, Lactate dehydrogense, Esterase

曹伟;姜洪杰;陈佩惠;杨连雪. 猪蛔虫与人蛔虫体壁蛋白组分可溶性抗原及同功酶的电泳分析[J]. 首都医科大学学报, 1988, 9(2): 109-113.

Cao wei. Electrop Horetic Analysis on Protein Components, Soluble Antigenic Preparation and Isoenzyme of Body Wall Of Ascaris Suum and Ascaris Lumpricoids[J]. Journal of Capital Medical University, 1988, 9(2): 109-113.

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