间接免疫过氧化物酶试验对侵袭性阿米巴病的诊断价值

首都医科大学学报 ›› 1988, Vol. 9 ›› Issue (3): 198-201.

间接免疫过氧化物酶试验对侵袭性阿米巴病的诊断价值

郭增柱, 王正仪, 桂淑环, 马伊莎

北京热带医学研究所

收稿日期:1987-01-01 修回日期:1900-01-01 出版日期:1988-07-15 发布日期:1988-07-15

Applying Indirect Immunoperoxidase Assay for Diagnosing Amebiasis

Guo Zengzhu

Beijing Tropical Medicine Research Institute

Received:1987-01-01 Revised:1900-01-01 Online:1988-07-15 Published:1988-07-15

摘要/Abstract

摘要： 以金黄色葡萄球菌A蛋白作为第二抗体,应用间接免疫过氧化物酶试验(IIP)检测79例阿米巴病患者的血清阿米巴抗体。58例肠外阿米巴病病人均呈阳性反应,滴度分布为1:128～1:4096;21例阿米巴痢疾患者亦全部阳性,滴度分布为1:64～1:4096,对照组中有一例肝癌患者呈阳性反应(1:64)。本法不如ELISA敏感,但由于无需阿米巴纯培养,更适于在国内推广应用。

关键词: 阿米巴病, 免疫诊断, 间接免疫过氧化物酶试验, 金黄色葡萄球菌A蛋白

Abstract: Indirect immunoperoxidase assay (IIP) technique was used to detect specific antibodies in sera of 79 cases of acute amebiasis. The second antibody used in the test consisted of horseradish peroxidase conjugated to protein A. Fifty eight cases of amebic liver abscess tested all showed positive reaction to IIP, and the titre ranged from 1:128 to 1:4096; 21 cases of acute amebic dysentery tested were also all positive but the titre ranged from 1:64 to 1:4096 only. One patient of liver cancer in the control series (52 cases) showed a mild positive reaction (1:64). IIP assay was not as sensitive as ELISA, but it does not require the use of axenic culture of E.histolytica.

Key words: amebiasis, immunodiagnosis, indirect immunoperoxidase assay (IIP) Protein A

郭增柱;王正仪;桂淑环;马伊莎. 间接免疫过氧化物酶试验对侵袭性阿米巴病的诊断价值[J]. 首都医科大学学报, 1988, 9(3): 198-201.

Guo Zengzhu. Applying Indirect Immunoperoxidase Assay for Diagnosing Amebiasis[J]. Journal of Capital Medical University, 1988, 9(3): 198-201.