首都医科大学学报 ›› 1988, Vol. 9 ›› Issue (4): 249-253.

• 论著 • 上一篇    下一篇

大鼠动情周期和妊娠早期子宫内膜组织化学的研究

史小林   

  1. 首都医学院组织胚胎学教研室
  • 收稿日期:1988-06-01 修回日期:1900-01-01 出版日期:1988-10-15 发布日期:1988-10-15

A Histochemical Study on the Changes of the Endometrium During the Estrus Cycle and Early Pregnancy in Rats

Shi Xiaolin   

  1. Department of Histology, Capital Institute of Medicine
  • Received:1988-06-01 Revised:1900-01-01 Online:1988-10-15 Published:1988-10-15

摘要: 实验观察了大鼠动情周期和妊娠第3、6、8天子宫表面上皮和腺上皮中糖原、脂类、碱性磷酸酶、酸性磷酸酶、葡萄糖-6-磷酸酶、非特异性酯酶、琥珀酸脱氢酶和3-β-羟基甾体脱氢酶等的组织化学反应。将糖原、脂类和酶活性的反应强度分为4级。结果发现:在间期,酸性磷酸酶、葡萄糖-6-磷酸酶和非特异性酯酶活性最低。糖原和脂类的含量也较低。在动情期的表面上皮中,非特异性酯酶、酸性磷酸酶和琥珀酸脱氢酶的活性增强。提示动情期内膜的糖代谢和脂类代谢开始旺盛。妊娠期,特别是妊娠第6天,糖原和脂类的含量以及酸性磷酸酶、葡萄糖-6-磷酸酶、非特异性酯酶的活性都明显增强,提示胚胎植入所需要的营养物质可能主要来源于子宫表面上皮的糖代谢和脂类代谢。在妊娠第6天表面上皮和腺上皮中碱性磷酸酶活性最低。另外还发现大鼠子宫表面上皮中含有3-β-羟基甾体脱氢酶,其意义有待探讨。

关键词: 子宫内膜, 组织化学, 大鼠

Abstract: Histochemical changes of the superficial epithelium and the glandular epithelium during estrus cycle and early pregnancy in rats were investigated. The results indicated that during diestrus the activity of ACP, G6Pase, NSE was declined and the amounts of glycogen and lipid were quite low as well. During estrus the activity of NSE, ACP and SDH was increased, which suggested that the metabolism of carbohydrate and lipid became very active. At the 6th day of rat pregnancy the amounts of glycogen and lipid and the activity of ACP, G6Pase, NSE were clearly raised, which suggested that the nutrition of blastocyst during implantation probably provided by the superficial epithelium. In addition, the superficial epithelium showed positive reaction to 3-β-HSDH, but its significance remains to be studied.

Key words: endometrium, histochemistry, rat