用透射电镜和免疫电镜的方法研究人类乳头瘤病毒与尖锐湿疣的关系

首都医科大学学报 ›› 1989, Vol. 10 ›› Issue (4): 237-241.

用透射电镜和免疫电镜的方法研究人类乳头瘤病毒与尖锐湿疣的关系

孟静, 高天祥

首都医学院电镜室

收稿日期:1989-02-10 修回日期:1900-01-01 出版日期:1989-10-15 发布日期:1989-10-15

Study of the Relation between Human Papillomavirus and Condyloma Acuminata with Electron Microscopy and Immuno-electron Microscopy

Meng Jing, Gao Tianxang

Laboratory of Electron Microscopy, Capital Institute of Medicine

Received:1989-02-10 Revised:1900-01-01 Online:1989-10-15 Published:1989-10-15

摘要/Abstract

摘要： 用透射电镜观察了30例尖锐湿疣的超微病理变化。多数尖锐湿疣存在空泡样变、双核、异型核、基质团及大量肿胀线粒体现象。但没有找到成熟的HPV颗粒。空泡样变可能是肿胀的线粒体破裂或胞浆基质团损耗所致。用金标免疫电镜法检出尖锐湿疣的HPV抗原阳性率为60%,而用酶标免疫电镜法其阳性率为47%。前法较后法敏感。大多数HPV抗原阳性细胞位子颗粒层和角化层。

关键词: 人类乳头瘤病毒, 尖锐湿疣, 免疫电镜

Abstract: 30 cases of condyloma acuminata were studied with electron microscopy and immuno-electronmicroscopy. Ultrastructral changes of HPV infection(koilocytosis, binucleation, giant irregular nuclei, electron dense matrix and inoreased number of swollen mitochondria) were present in high prevalence in condyloma acuminata. But no mature HPV particles were found. The koilocytosis may result from rupturing of swollen mitochondria and worn-out cytoplasmic dense matrix. Expression of HPV antigen was shown in 60% cases with gold-staining immuno-etectronmicroscopy, and in 47% cases with enzyme-staining immuno-electronmicroscopy. Therefore, the former method proved to be more sensitive than the latter one. The majority of HPV antigen possitive cells were located in the stratum granulosum and the stratum corneum.

Key words: HPV, condyloma acuminata, immuno-electronmicroscopy

孟静;高天祥. 用透射电镜和免疫电镜的方法研究人类乳头瘤病毒与尖锐湿疣的关系[J]. 首都医科大学学报, 1989, 10(4): 237-241.

Meng Jing;Gao Tianxang. Study of the Relation between Human Papillomavirus and Condyloma Acuminata with Electron Microscopy and Immuno-electron Microscopy[J]. Journal of Capital Medical University, 1989, 10(4): 237-241.

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