生物素标记组合探针杂交检测患儿尿液以诊断巨细胞病毒感染

首都医科大学学报 ›› 1991, Vol. 12 ›› Issue (1): 36-40.

生物素标记组合探针杂交检测患儿尿液以诊断巨细胞病毒感染

李元浩¹, 戴斌¹, 梁承玮², 关碧琰²

1. 中国药品生物制品检定所;2. 北京市天坛医院儿科

收稿日期:1989-11-21 修回日期:1900-01-01 出版日期:1991-01-15 发布日期:1991-01-15

Detection of Clinical Human Cytomegalovirus Infection from Children by DNA Hybridisation with the Biotinylated Combined Probes

Li Yuanhao¹, Dai Bin¹, Liang Chengwei², Guan Biyan²

1. National Institute for the Control of Pharmaceutical and Biological Products, Beijing;2. Beijing Tiantan Hospital

Received:1989-11-21 Revised:1900-01-01 Online:1991-01-15 Published:1991-01-15

摘要/Abstract

摘要： 利用HCMV AD 169株的克隆DNA片段制成生物素标记的组合探针进行临床标本的HCMV DNA的杂交检测,此探针特异性强,不与其它类DNA发生非特异性杂交,可检出25pg同源DNA,100～500个HCMV感染细胞或2.57LgTCID₅₀ HCMV病毒悬液.杂交法结果与病毒分离结果符合率极高.初步用于临床尿标本检测得出,先天畸型,乳儿肝炎及激素治疗三组患儿中,HCMV的排毒率分别为100%(3/3),61%(11/18)和75%(3/4).提示HCMV可能是临床部分患儿的重要致病源.同时表明杂交法对HCMV感染的检测是快速、特异及敏感的.

关键词: HCMV, 尿液, 生物素组合探针

Abstract: A dignostic assay has been developed to detect HCMV DNA in clinical specimens with Biotin-labelled combined probes which were prepared from some cloned fragments of HCMV strain AD169.The labelled probe can detect 25pg of HCMV homologous DNA and fail to hybridize with DNA from other sources.With excellent specificity,the combined DNA probes can detect 100～500 infected cells or 2.57lg TCID₅₀ of HCMV.The assay have high correlation when compared to virus isolation.In the detection of clinical speciments,it correctly identified 3(100%) of 3 urine specimens from congenital deformith positive children.11(61%) of 18 urine specimens from hepatitis positive baby,and 3(75%) of 4 urine specimens from Hormon treatment positive children.There is the evidence that the hybridization assay are specific,sensitive and rapid method for HCMV infection detection.

Key words: HCMV, Urine, biotinylatied combined probes

李元浩;戴斌;梁承玮;关碧琰. 生物素标记组合探针杂交检测患儿尿液以诊断巨细胞病毒感染[J]. 首都医科大学学报, 1991, 12(1): 36-40.

Li Yuanhao;Dai Bin;Liang Chengwei;Guan Biyan. Detection of Clinical Human Cytomegalovirus Infection from Children by DNA Hybridisation with the Biotinylated Combined Probes[J]. Journal of Capital Medical University, 1991, 12(1): 36-40.

[1]	王巍伟, 王珊珊, 张曼. Kininogen 1在非小细胞肺癌体液和组织中的表达及临床意义[J]. 首都医科大学学报, 2021, 42(1): 77-82.
[2]	王玲玲, 陈桂欣, 王朝辉, 孙赛男, 孙博谦, 李威. 脑梗死患者尿液11脱氢血栓烷B2与风险因素交互作用对疾病发生的影响[J]. 首都医科大学学报, 2019, 40(5): 721-725.
[3]	栾海霞, 袁慧, 贺建勋, 马旭, 姚兴荣, 曾小莉, 桂媛, 刘玉磊. 尿NGAL不同时点浓度变化在心脏术后急性肾损伤的早期诊断价值[J]. 首都医科大学学报, 2015, 36(4): 592-596.
[4]	申哲洙;顾晋;赵威;张志谦. 尿液中MMP-2、MMP-9及MMP-9/NGAL复合物的表达在乳腺癌筛查中的意义[J]. 首都医科大学学报, 2008, 29(3): 395-398.
[5]	岳秀玲;李玉文;周金;马骏龙;张时民;丛玉隆. UF-100检测北京地区正常人群尿液有形成分的调查[J]. 首都医科大学学报, 2007, 28(5): 632-635.