首都医科大学学报 ›› 1993, Vol. 14 ›› Issue (2): 124-127.

• 论著 • 上一篇    下一篇

用国产羟基磷灰石纯化抗恙虫立克次体单克隆抗体

张惠君, 陈燕, 黄松如, 黄敏君   

  1. 北京市热带医学研究所
  • 收稿日期:1992-03-25 修回日期:1900-01-01 出版日期:1993-04-15 发布日期:1993-04-15

Purification of Monoclonal Antibodies Against Rickettsia Tsutsugamushi by Using China-Made Hydroxyl apatite Chromatography

Zhang Huijun, Chen Yan, Huang Songru, Huang Minjun   

  1. Beijing Tropical Medicine Research Institute
  • Received:1992-03-25 Revised:1900-01-01 Online:1993-04-15 Published:1993-04-15

摘要: 用国产羟基磷灰石层析柱分别提纯含抗恙虫立克次体单克隆抗体 Kp3(IgG1)及 Kp4(IgM)的小鼠腹水,回收率较用蛋白 A 琼脂糖层析或 DEAE-纤维素离子交换层析为高。且抗体活性稳定.

关键词: 恙虫立克次体, 单克隆抗体, 羟基磷灰石

Abstract: Ascites of BALB/c mice containing monoclonal antibodies Kp3 (IgG1)and Kp4(IgM) against Rickettsia tsutsugamushi were purified respectively by using China-made hydroxyla-patite chromatography.The rate of reclamation of this method was higher than that obtained by chromatography on protein A-sepharose and ion exchange chromatography on DEAE cel-lulose.The antibody activities of the purified monoclonal antibodies were stable.This method can be used to purify both IgG and IgM.

Key words: Rickettsia tsutsugamushi, monoclonal antibody, hydroxylapatite

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