首都医科大学学报 ›› 1993, Vol. 14 ›› Issue (3): 188-191.

• 论著 • 上一篇    下一篇

内皮细胞生长因子的分离和纯化

肖蓬, 徐燕玲, 王保君, 张景艳, 东博涛, 温绍君   

  1. 首都医学院宣武医院 北京老年病医疗研究中心细胞室
  • 收稿日期:1993-01-18 修回日期:1900-01-01 出版日期:1993-07-15 发布日期:1993-07-15

Purification of Endothelial Cell Growth Factor

Xiao Peng, Xu Yanling, Wan Baojun, Zhang Jingyan, Dong Botao, Wen Shaojun   

  1. Xuanwu Hospital, Capital Institute of Medicine Beijing Geriatric Clinical &, Research Center Department of Cytologe
  • Received:1993-01-18 Revised:1900-01-01 Online:1993-07-15 Published:1993-07-15

摘要: 参照 Lobb 和 Fett 方法,用肝素亲和层析法从牛脑组织中提取出纯度较高的内皮细胞生长因子,后者能明显促进体外人脐静脉内皮细胞增殖。

关键词: 肝素亲和层析法, 牛脑, 内皮细胞生长因子, 分离和纯化

Abstract: According to the Lobb and Fett's mehtod,endothelial cell growth factor has been puri-fied homogeneously from bovine brain by heparin affinity chromatograph in our labratory. The results of culturing human umbilical vein endothelial cells show that our purifed en-dothelial cell growth factor is highly effective in stimulating the growth of endothelial cells.

Key words: endothelial cell growth factor, purification, heparin affinity chromatograph, bovin brain

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