对流免疫电泳、一维与二维电泳免疫印迹检测ENA抗体

首都医科大学学报 ›› 1993, Vol. 14 ›› Issue (4): 280-283.

对流免疫电泳、一维与二维电泳免疫印迹检测ENA抗体

俞乃昌, 王红, 孙颖, 高常青

首都医学院宣武医院内科免疫室

收稿日期:1992-07-05 修回日期:1900-01-01 出版日期:1993-10-15 发布日期:1993-10-15

A Study of ENA Antibodies with Counterimmuno-electrophoresis(CIE),One and Two Dimensional Electrophoresis and Immunoblotting(1D-IB,2D-IB)

Yu Naichang, Wang Hong, Sun Ying, Gao Changqing

Xuanwu Hospital, Capital Institute of Medicine

Received:1992-07-05 Revised:1900-01-01 Online:1993-10-15 Published:1993-10-15

摘要/Abstract

摘要： 用小牛胸腺、人脾脏、HEP-2细胞为抗原作对流免疫电泳,用HEP-2细胞为抗原作一维(1D-IB)与二维电泳免疫印迹(2D-IB)。检测20例血清中的ENA抗体。ID-IB唯独对检测Sm抗体比CIE敏感,2D-IB可有效地检出SS-A、SS-B抗体并精细地分析其特异性,也能检出68、20kD的RNP抗体,但在20例SLE血清中仅检测出3例Sm抗体。以HEP-2细胞为抗原作CIE,SS-B抗体的检出很低(2/20).

关键词: 电泳, 结缔组织疾病, 系统性红斑琅疮

Abstract: Twenty SLE sera were examined for ENA autoantibodies with calf thymus and human spleen extracts,and HEP-2 cell lysate as antigens in CIE,and HEP-2 lysate as antigen in 1D-IB and 2D-IB.1D-IB was more sensitive than CIE for the detection of Sm antibody only. 2D-IB could effectively detect SS-A and SS-B antibodies and analyze their fine specificities; it could demonstrate RNP antibodies reacting with 68 kD and 20 kD polypeptides,but had a low detection rate for Sm antibody(3 positives out of 20 sera).The use of HEP-2 cell lysate as antigen in CIE yielded a low detection rate for SS-B antibody(2/20).

Key words: electropholysis, connective tissue disease, systemic lupus erythematosus

中图分类号:

R593.241

俞乃昌;王红;孙颖;高常青. 对流免疫电泳、一维与二维电泳免疫印迹检测ENA抗体[J]. 首都医科大学学报, 1993, 14(4): 280-283.

Yu Naichang;Wang Hong;Sun Ying;Gao Changqing. A Study of ENA Antibodies with Counterimmuno-electrophoresis(CIE),One and Two Dimensional Electrophoresis and Immunoblotting(1D-IB,2D-IB)[J]. Journal of Capital Medical University, 1993, 14(4): 280-283.

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