鳀鱼鱼油中EPA和DHA的气相色谱分析

首都医科大学学报 ›› 1994, Vol. 15 ›› Issue (1): 21-25.

鳀鱼鱼油中EPA和DHA的气相色谱分析

燕福生, 李鸿筠, 朱冬生

首都医学院化学教研室

收稿日期:1993-04-19 修回日期:1900-01-01 出版日期:1994-01-15 发布日期:1994-01-15

Gas Chromatographic Analysis for EPA and DHA in the Anchovy Oil

Yan Fusheng, Li Hongjun, Zhu Dongsheng

Department of Chemistry, Capital Institute of Medicine

Received:1993-04-19 Revised:1900-01-01 Online:1994-01-15 Published:1994-01-15

摘要/Abstract

摘要： 用气相色谱法测定鳀鱼鱼油中EPA、DHA的含量,鱼油PUFA用0.5mol/L NaOH-甲醇液皂化,BF₃-甲醇酯化成脂肪酸甲酯,进行气相色谱测定.内标物用TSA-M.模拟试验5份平均回收率EPA-M为89.86%,DHA-M为89.78%.变异系数EPA-M为0.80%,DHA-M为0.64%.表明本法简便、快速、重现性好.

关键词: 气相色谱分析, 鳀鱼鱼油, EPA, DHA

Abstract: In this paper the method of gas chromatographic(GC)analysis for eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)in the anchovy oil was reported. The polyunsaturated fatty acids(PUFA)in the fish oil were saponified with 0.5 mol/L NaOH-CH₃OH, and esterified with BF₃-CH₃OH for GC analysis. The quantity was determined by internal standard method with TSA-M as a reference. In five determinations, the average rate of reclamation for EPA-M and DHA-M was 89.86%, 89.78% respectively. The variation coefficient for EPA-M was 0.80%, and for DHA-M was 0.64%. The proposed method is simple, rapid and reproducible.

Key words: gas chromatographic analysis, anchovy oil, EPA, DHA

中图分类号:

燕福生;李鸿筠;朱冬生. 鳀鱼鱼油中EPA和DHA的气相色谱分析[J]. 首都医科大学学报, 1994, 15(1): 21-25.

Yan Fusheng;Li Hongjun;Zhu Dongsheng. Gas Chromatographic Analysis for EPA and DHA in the Anchovy Oil[J]. Journal of Capital Medical University, 1994, 15(1): 21-25.