神经节苷脂制备中溶剂效率的研究

首都医科大学学报 ›› 1994, Vol. 15 ›› Issue (4): 270-274.

神经节苷脂制备中溶剂效率的研究

潘颖, 黄如彬, 王泽生, 佟大山, 史小苓

首都医学院生物化学教研室

收稿日期:1993-12-09 修回日期:1900-01-01 出版日期:1994-10-15 发布日期:1994-10-15

The Study of Solvent Efficiency in Ganglioside Preparation

Pan Ying, Huang Rubin, Wang Zesheng, Tong Dashan, Shi Xiaoling

Department of Biochemistry, Capital Institute of Medicine

Received:1993-12-09 Revised:1900-01-01 Online:1994-10-15 Published:1994-10-15

摘要/Abstract

摘要：

神经节苷脂在组织中的含量很低,分离、提取过程极其复杂,有机溶剂消耗量非常大.利用回收的有机溶剂,结合离心液相色谱技术,使有机溶剂用量下降了88.5%,组织提取率达到0.0894%.

关键词: 神经节苷脂, 溶剂, 离心液相色谱

Abstract:

Gangliosides(Gls)content is very low in organism. The complex procedures of isolation and purification of Gls expended large amount of solvent. The technique of the centrifugal liquid chromatography and recovery of waste solvent were effective steps improved solvent efficiency in Gls preparation. The consumption of solvent went down by 88. 5% from 17.5L.The productivity of pig brain Gls was 0.0894%.

Key words: gangliosides(Gls), solvent, centrifugal liquid chromatography(CLC)

中图分类号:

Q45.3

潘颖;黄如彬;王泽生;佟大山;史小苓. 神经节苷脂制备中溶剂效率的研究[J]. 首都医科大学学报, 1994, 15(4): 270-274.

Pan Ying;Huang Rubin;Wang Zesheng;Tong Dashan;Shi Xiaoling. The Study of Solvent Efficiency in Ganglioside Preparation[J]. Journal of Capital Medical University, 1994, 15(4): 270-274.

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