从家兔分离贾第虫并建立纯培养

首都医科大学学报 ›› 1995, Vol. 16 ›› Issue (1): 24-26.

从家兔分离贾第虫并建立纯培养

阎歌, 卢思奇, 王凤芸

首都医学院寄生虫学教研室

收稿日期:1993-10-08 修回日期:1900-01-01 出版日期:1995-01-15 发布日期:1995-01-15

Isolation and Axenic Cultivation of Giardia lamblia from the Feces of Rabbit

Yan Ge, Lu Siqi, Wang Fengyun

Deparrment of Parasitology, Capital institute of Medicine

Received:1993-10-08 Revised:1900-01-01 Online:1995-01-15 Published:1995-01-15

摘要/Abstract

摘要： 浓集、纯化兔粪便中的贾第虫包囊,用生理盐水制成悬液,感染BABL/C乳鼠。接种后第8d,在无菌条件下从鼠小肠上段分离滋养体,接种于改良TYI-S-33培养基内,置37℃培养。培养后第20d虫体在管壁形成密集的细胞单层,传代20余次,并经冷冻及复苏,虫体均生长良好。

关键词: 贾第虫, BABL／C乳鼠, 纯培养, 改良TYI-S-33培养基

Abstract: Cysts of Giardia lamblia isolated from the feces of a Giardia-infected rabbit were inoculated into suckling BABL/C mice.Trophozoites of G. Lamblia obtained from the intestinal lumen of infected BABL/C mice were cultivated in modified TYI-S-33 medium at 37℃.Trophozoites grew luxuriantly and formed an intensive monolayer on the inner surface of the culture tube on day 20 after initial cultivation. Periodic examinations of Giardia culture for bacteria contamination used with petri dishs containing blood agar, proved negation. This axenic culture has been made more than 20 subcultures.

Key words: Giardia lamblia, BABL/C suckling mices: axenic cultivation, modified TYI-S-33 medium

中图分类号:

R382.2

阎歌;卢思奇;王凤芸. 从家兔分离贾第虫并建立纯培养[J]. 首都医科大学学报, 1995, 16(1): 24-26.

Yan Ge;Lu Siqi;Wang Fengyun. Isolation and Axenic Cultivation of Giardia lamblia from the Feces of Rabbit[J]. Journal of Capital Medical University, 1995, 16(1): 24-26.

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