用免疫组织化学方法观察寒证及温热药治疗后大鼠垂体激素细胞的变化

首都医科大学学报 ›› 1995, Vol. 16 ›› Issue (1): 7-10.

用免疫组织化学方法观察寒证及温热药治疗后大鼠垂体激素细胞的变化

李良¹, 周以健¹, 温祥云¹, 宋辉², 梁月华²

1. 首都医学院病理解剖学教研室;2. 北京医科大学

收稿日期:1993-12-11 修回日期:1900-01-01 出版日期:1995-01-15 发布日期:1995-01-15

Immunohistochemical Changes of Pituitary Hormone-Secreting Cells in Rats with "Cold" Syndrome Treated by Herb Medicine with Heat Nature

Li Liang¹, Zhou Yijian¹, Wen Xiangyun¹, Song Hui², Liang Yuehua²

1. Dept. of Pathology, Capital Insitute of Medicine;2. Beijing Medical University

Received:1993-12-11 Revised:1900-01-01 Online:1995-01-15 Published:1995-01-15

摘要/Abstract

摘要：

用复方温热药(热Ⅰ:附子、干姜,热Ⅱ:党参、黄芪)治疗寒证大鼠。取大鼠垂体,用ABC免疫组化方法,观察促黄体激素、促甲状腺激素和促肾上腺皮质激素细胞的变化。结果表明:寒证时,3种垂体激素均有明显增高,给予热药治疗后各垂体激素大量释放。其中以ACTH释放尤为显着。两种复方温热药作用相同。通过对3种激素的靶器官(睾丸、甲状腺、肾上腺)的形态学观察,均未发现有明显改变,提示寒凉及温热药的剂量及给药时间尚未引起其靶器官的器质性改变。

关键词: 寒证, ACTH细胞, TSH细胞, LH细胞, 免疫组织化学

Abstract:

The experimental rats were fed with cold-natured medicines (Zhimu, Shigao) for three weeks to made a "cold " syndrome model. After that, they were treated with two kinds of heat-natured medicines (I-Fuzi, Ganjiang: Ⅱ-Dangshen, Huangqi) respectively. After 5 and 7 days , the pituitary, thyroid, adrenal, and testis were taken.The specific anti-LH, anti-TSH adn anti-ACTH sera were used in the avidin-biotin-complex (ABC) immunohistochemical method to observe the changes of ACTH, LHand TSHcells. It was observed that the immunohistochemical reaction of the three kinds of pituitary hormone-secreting cells increased obviously in rats with "cold" syndrome and weakened after treated with heat-natured medicines. Among the three kinds of cells the change of ACTH cell was most remarkable.The changes of pituitary hormone-secreting cells treated with heat-natured medicines Iwere similar to those of heat-natured medicines Ⅱ. We also observed the morphologic changes of the three target organs. It was found that there was no obvious change in them and it might indicate that the amount of hormone was not enough to cause morphologic changes but only the functional ones.

Key words: cold syndrome, ACTH cell, TSH cell, LH cell, immunohistochemistry

中图分类号:

R392.31

李良;周以健;温祥云;宋辉;梁月华. 用免疫组织化学方法观察寒证及温热药治疗后大鼠垂体激素细胞的变化[J]. 首都医科大学学报, 1995, 16(1): 7-10.

Li Liang;Zhou Yijian;Wen Xiangyun;Song Hui;Liang Yuehua. Immunohistochemical Changes of Pituitary Hormone-Secreting Cells in Rats with "Cold" Syndrome Treated by Herb Medicine with Heat Nature[J]. Journal of Capital Medical University, 1995, 16(1): 7-10.

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