带有酪氨酸羟化酶基因的pHSV1TH质粒构建

首都医科大学学报 ›› 1996, Vol. 17 ›› Issue (2): 83-88.

带有酪氨酸羟化酶基因的pHSV₁TH质粒构建

孙阳, 杨慧, 鲁瑛, 李淑婷, 田竟生, 徐群渊

首都医科大学北京神经科学研究所

收稿日期:1996-03-29 修回日期:1900-01-01 出版日期:1996-04-15 发布日期:1996-04-15

Reconstruction of Plasmid PHSV₁TH,A Vector Containing Tyrosine Hydroxylase Gene

Sun Yang, Yang Hui, Lu Ying, Li Shuting, Tian Jingsheng, Xu Qunyuan

Beijing Institute for Neurosciences, Capital University of Medical Sciences

Received:1996-03-29 Revised:1900-01-01 Online:1996-04-15 Published:1996-04-15

摘要/Abstract

摘要：

为高效表达酪氨酸羟化酶(TH)基因,采用分子克隆技术,构建以单纯疱疹病毒Ⅰ型-胸苷激酶(HSV₁-TK)基因为启动子的TH基因的重组质粒,以了解在HSV₁-TK启动子作用下TH基因的表达水平。为巴金森病基因治疗提供手段。

关键词: 酪氨酸羟化酶基因, 单纯疱疹病毒Ⅰ型-胸苷激酶基因, DNA质粒重组, pHSV₁TH质粒

Abstract:

For efficent expression of the tyrosine hydroxylase (TH) gene, the molecular cloning was used to construct a vector containing the TH gene and a promoter,the herpes simplex virus type Ⅰ-thymidine kinase (HSV1-TK).The promoter was from the plasmid,pHSV-106 and the TH gene from the plasmid PKSTH.The newly reconstructed plasmid PHSV1TH was identified by analysis of restriction endonuclease and \.The results indicated that the HSV1-TH plasmid would be a useful tool for gene therapy of Parkinson' s disease.

Key words: tyrosine hydroxylase gene, herpes simplex virus type Ⅰ-thymidine kinase gene, DNA plasmid reconstruction, PHSV1 TH plasmid

中图分类号:

Q785

孙阳;杨慧;鲁瑛;李淑婷;田竟生;徐群渊. 带有酪氨酸羟化酶基因的pHSV₁TH质粒构建[J]. 首都医科大学学报, 1996, 17(2): 83-88.

Sun Yang;Yang Hui;Lu Ying;Li Shuting;Tian Jingsheng;Xu Qunyuan. Reconstruction of Plasmid PHSV₁TH,A Vector Containing Tyrosine Hydroxylase Gene[J]. Journal of Capital Medical University, 1996, 17(2): 83-88.

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带有酪氨酸羟化酶基因的pHSV₁TH质粒构建

Reconstruction of Plasmid PHSV₁TH,A Vector Containing Tyrosine Hydroxylase Gene

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