TA98及其衍生菌株检测大气颗粒物致突变作用的比较研究

首都医科大学学报 ›› 1996, Vol. 17 ›› Issue (4): 263-266.

TA98及其衍生菌株检测大气颗粒物致突变作用的比较研究

孔祥环¹, 尹学钧¹, 王虹¹, 肖中新¹, 褚金花¹, 李向红²

1. 首都医科大学环境卫生学教研室;2. 首都医科大学数学教研室

收稿日期:1995-09-13 修回日期:1900-01-01 出版日期:1996-10-15 发布日期:1996-10-15

Comparison of Mutagenicity of Airborne Particulates for Strain TA98 with that for its New Derivative Strains

Kong Xianhuan¹, Yin Xuejue¹, Wang Hong¹, Xiao Zhongxin¹, Chu Jinhua¹, Li Xianghong²

Department of Environmental Health, Capital University of Medical Sciences

Received:1995-09-13 Revised:1900-01-01 Online:1996-10-15 Published:1996-10-15

摘要/Abstract

摘要： 应用TA98及其衍生物,检测了沈阳市不同功能分区大气悬浮颗粒提取物的致突变性,并对各菌株进行了比较。结果显示,在无S9存在时,颗粒提取物对菌株YG1024、YG1021及TA98的直接致突变作用高于菌株TA98NR、TA98/1,8DNP6,并以YG1024升高最明显。提示颗粒提取物含有NO₂-PAHs,其中硝基芘类,特别是二硝基芘类是该颗粒物中主要直接致突变物。YG菌株,特别是YG1024在检测含有NO₂-PAHs的环境样本中的致突变性更为敏感。实验结果提示沈阳市工业区、居民区污染严重。

关键词: 大气悬浮颗粒物, 致突变性, Ames试验, 硝基多环芳烃

Abstract: The mutagenicity of airborne particulates from differential function districts in Shenyang was studied with salmonella typhimurium strain TA98, the classically beliered sensitive strain, and its new derivative strains TA98NR, TA98/1 ,8DNP6, YG 1021 and YG1024,and sensitivity of these new strains were compared with that of TA98.The results indicated that the mutagenicity of samples for YG1024,YG1021 and TA98 were significantly stronger than that for TA98NR and TA98/1, 8DNP₆.Strain YG1024 was more specific to the mutagenicity of samples than TA98.This strongly suggests the presence of nitrated polycyclic aromatic hydro-carbons (NO₂-PAHs)in these particulates and that nitropyrenes must be the major direct-acting mutagens, especically dinitropyrenes (DNPs) .YG1024 can become the ralatively sensitive indicating strain in detecting mutagenicity of samples of environment.The results indicated samples from industrial and residential districts contained more NO₂-PAHs than that from other districts.

Key words: airborne particulate, mutagenicity, Ames test, nitrated polycyclic aromatic hydrocarbons ( NO₂-PAHs)

中图分类号:

R122

孔祥环;尹学钧;王虹;肖中新;褚金花;李向红. TA98及其衍生菌株检测大气颗粒物致突变作用的比较研究[J]. 首都医科大学学报, 1996, 17(4): 263-266.

Kong Xianhuan;Yin Xuejue;Wang Hong;Xiao Zhongxin;Chu Jinhua;Li Xianghong. Comparison of Mutagenicity of Airborne Particulates for Strain TA98 with that for its New Derivative Strains[J]. Journal of Capital Medical University, 1996, 17(4): 263-266.

[1]	尹学钧;肖中新;褚金花;孔祥环;王虹;刘君卓. 对液化石油气燃烧产物接触者尿致突变性的研究[J]. 首都医科大学学报, 1996, 17(1): 20-23.
[2]	孔祥环;蒋保季;王惠琴;尹学钧;唐玉萍;马忠杰;沈家琴;张宏伟;肖中新. 发酵培育冬虫夏草毒理研究　Ⅱ．致突变性研究[J]. 首都医科大学学报, 1995, 16(4): 256-258.