实验性脾虚证大鼠肾上腺形态学与细胞化学研究

首都医科大学学报 ›› 1997, Vol. 18 ›› Issue (1): 25-28.

实验性脾虚证大鼠肾上腺形态学与细胞化学研究

季凤清¹, 王秀琴¹, 史小林², 路欣², 许晴²

1. 首都医科大学组织胚胎教研室;2. 首都医科大学生殖医学研究室

收稿日期:1996-10-20 修回日期:1900-01-01 出版日期:1997-01-15 发布日期:1997-01-15

The Morphological and Cytochemical Study on Adrenal Glands of Rats with Experimental "Splenic Deficiency" Syndrome

Ji Fengqing¹, Wang Xiuqin¹, Shi Xiaolin², Lu Xin², Xu Qing²

1. Department of Histology and Embryology, Capital University of Medical Sciences;2. Department of Reproductive Medicine, Capital University of Medical Sciences

Received:1996-10-20 Revised:1900-01-01 Online:1997-01-15 Published:1997-01-15

摘要/Abstract

摘要：

对实验性脾虚证和对照组大鼠的肾上腺进行常规组织学与细胞化学的对比观察。结果显示:脾虚组肾上腺球状带明显变窄;各带细胞染色浅,胞质呈空泡状;细胞间隙变宽。细胞化学观察显示肾上腺皮质与髓质琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)、碱性磷酸酶(ALP)、酸性磷酸酶(ACP)、三磷酸腺苷酶(ATPase)、3-β-羟甾脱氢酶(3-β-HSDH)、糖原(PAS)均有不同程度的改变,表现明显的有ACP和ATPase。研究结果表明脾虚证不仅表现为消化系统功能低下,肾上腺也有明显的病理改变。

关键词: 实验性脾虚证, 大鼠肾上腺, 细胞化学

Abstract:

Twenty adult wistar rats were used and divided into two groups,normal control group and "experimental splenic" deficiency group. The experimental rats had been fed with the decoction of Dahuang for 42 d with ensuing experimental "splenic deficiency" symptom.The adrenal glands of two groups were observed morphologically and cytochemically.The resulted morphological study of experimental "splenic deficiency" group indicated that the zona glomernlosa became narrower, the stains in every zones were light, the cytoplasm were vesicular vacuolated, the cell interstitium became wider. The cytochemical study showed that activity of every enzyme in cortex and medulla of adrenal glands changed in various degrees and ACP, ATPase showed definite changes.This research proved that "splenic deficiency" syndrome resulted in not only that the function of digestive system became lowered, but also that the adrenal gland underwent pathological changes.

Key words: experimental "spleen deficiency", syndrome;adrenal gland of rat;cytochemistry

中图分类号:

Q492.4

季凤清;王秀琴;史小林;路欣;许晴. 实验性脾虚证大鼠肾上腺形态学与细胞化学研究[J]. 首都医科大学学报, 1997, 18(1): 25-28.

Ji Fengqing;Wang Xiuqin;Shi Xiaolin;Lu Xin;Xu Qing. The Morphological and Cytochemical Study on Adrenal Glands of Rats with Experimental "Splenic Deficiency" Syndrome[J]. Journal of Capital Medical University, 1997, 18(1): 25-28.

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