首都医科大学学报 ›› 1997, Vol. 18 ›› Issue (4): 310-312.

• 论著 • 上一篇    下一篇

2种制备抗新城鸡瘟病毒抗体方法的比较

柯岩, 陈哲生   

  1. 首都医科大学微生物学免疫学教研室
  • 收稿日期:1996-10-16 修回日期:1900-01-01 出版日期:1997-10-15 发布日期:2013-10-28

A Comparison between Two Methods for Preparing Antibodies Against Newcastle Virus

Ke Yan, Chen Zhesheng   

  1. Department of Microbiology and Immunology, Capital University of Medical Sciences
  • Received:1996-10-16 Revised:1900-01-01 Online:1997-10-15 Published:2013-10-28

摘要: 采用首次免疫加完全佐剂和不加完全佐剂2种方法,分别制备兔抗新城鸡瘟病毒抗体。结果显示:首次免疫加入完全佐剂的免疫方法产生的血凝抑制抗体效价为1/640,显著低于不加完全佐剂(血凝抑制抗体效价为1/2560)的免疫方法。提示:在制备抗病毒抗体中,应该选用首次免疫不加入完全佐剂的免疫方法。

关键词: 新城鸡瘟病毒, 完全佐剂, 血凝抑制抗体滴度

Abstract: Two methods for preparing rabbit type antibody against the New Castle Virus were reported,one being with Freund's complete adjuvant(FCA),the other without FCA.The result showed that the titer of hemagglutination inhibition antibody of the former was 1/640 while the latter was 1/2560. In conclusion,the method without FCAwas firstly selected for making rabbit type antibody against the New Castle Virus.

Key words: New Castle virus, Freund's complete adjuvant(FCA), hemagglutinatoin inhibition antibody titer

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