上皮源性恶性肿瘤患者p16/MTS1抑癌基因失活研究

首都医科大学学报 ›› 2000, Vol. 21 ›› Issue (1): 33-36.

上皮源性恶性肿瘤患者p16/MTS1抑癌基因失活研究

高居忠, 朱章菱, 肖白, 王跃, 迟闺珠, 于莎莎, 刘复兴, 李正廷, 张愚

首都医科大学附属北京红十字朝阳医院

收稿日期:1999-01-26 修回日期:1900-01-01 出版日期:2000-01-15 发布日期:2000-01-15

Inactivation of the p16/MTS1 Gene in Multiple Human Cancers

Gao Juzhong, Zhu Zhangling, Xiao Bai, Wang Yue, Chi Guizhu, Yu Shasha, Liu Fuxing, Li Zhengting, Zhang Yu

Beijing Red Cross Chaoyang Hospital, Affiliate of Capital University of Medical Sciences

Received:1999-01-26 Revised:1900-01-01 Online:2000-01-15 Published:2000-01-15

摘要/Abstract

摘要： 以不同种类的上皮源性恶性肿瘤患者为研究对象,分析肿瘤组织中p16/MTS1基因纯合缺失、异常甲基化和表达缺失。发现p16基因纯合缺失率为14%(15/108);异常甲基化检出率为14%(8/58);p16蛋白表达缺失率为44%(28/63);按组织学分级,中、低分化组p16蛋白表达缺失率显着高于高分化组(P<0.05).p16基因失活患者普遍预后差。肺癌、食管癌患者p16基因失活者17例,手术后6个月内6例死亡,1例转移;p16基因发生缺失和异常甲基化的6例膀胱癌患者中4例复发。研究结果表明,p16基因失活在上皮源性恶性肿瘤患者中是较为常见的基因变化,与患者的病理分级和预后有密切关系。

关键词: p16/MTS1抑癌基因, 上皮源性恶性肿瘤, 失活

Abstract: Inactivation of the p16/MTS1 gene was examined by homozygous deletion, methylation of 5′ CpG islands and silencing of expression in multiple tumor samples from patients. Homozygous deletion and de novo methylation of the gene were confirmed in 15(14%) of 108 and 8(14%) of 58 patients, respectively. Protein product of p16 was detected by immunohistochemical method. Rate of p16 gene expressive deletion was 44% (28/63). The p16 deletion rates were significantly higher in poorly differen tiated group. In accordance with following up the patients, 6 cases died and 1 case metastasized after operation in a half year in 17 patients with lung cancer or esophageal carcinoma whose p16 gene was inactivated; 4 cases recurred in 6 patients with bladder cancer whose p16 gene were homozygously deleted or methylated. These results suggested that inactivation of the p16 gene appeared to be a common event in multiple cancers and might be associated with histological grade and prognosis.

Key words: p16/MTS1 tumor suppressor gene, human cancers, inactivation

中图分类号:

R730.45

高居忠;朱章菱;肖白;王跃;迟闺珠;于莎莎;刘复兴;李正廷;张愚. 上皮源性恶性肿瘤患者p16/MTS1抑癌基因失活研究[J]. 首都医科大学学报, 2000, 21(1): 33-36.

Gao Juzhong;Zhu Zhangling;Xiao Bai;Wang Yue;Chi Guizhu;Yu Shasha;Liu Fuxing;Li Zhengting;Zhang Yu. Inactivation of the p16/MTS1 Gene in Multiple Human Cancers[J]. Journal of Capital Medical University, 2000, 21(1): 33-36.