中美猪型旋毛虫株不同发育阶段同工酶的电泳分析

首都医科大学学报 ›› 2000, Vol. 21 ›› Issue (1): 41-42.

中美猪型旋毛虫株不同发育阶段同工酶的电泳分析

张帆, 姜洪杰, 黄松, 诸欣平

首都医科大学寄生虫学教研室

收稿日期:1999-03-30 修回日期:1900-01-01 出版日期:2000-01-15 发布日期:2000-01-15

Electrophoresis Analysis on Isoenzyme of Different Development Stages of Chinese and American Trichinella spiralis

Zhang Fan, Jiang Hongjie, Huang Song, Zhu Xinping

Department of Parasitology, Capital University of Medical Sciences

Received:1999-03-30 Revised:1900-01-01 Online:2000-01-15 Published:2000-01-15

摘要/Abstract

摘要： 应用聚丙烯酰胺凝胶电泳(PAGE)和UVP凝胶分析仪系统,分析比较了中、美猪型旋毛虫成虫和幼虫的酯酶(EST)同工酶及乳酸脱氢酶(LDH)同工酶的带谱。结果表明：猪型黑龙江株旋毛虫成虫和幼虫的EST同工酶酶带均为2条,猪型美国株旋毛虫成虫和幼虫EST同工酶酶带分别为3条和2条;中、美猪型旋毛虫成虫的LDH同工酶无酶带,而幼虫则各具1条酶带,且其迁移率也不同。结果提示：来自不同地域的旋毛虫在遗传学上存在差异,同时,不同发育阶段同工酶酶谱也有明显不同。

关键词: 旋毛虫不同发育阶段, EST同工酶, LDH同工酶

Abstract: The studies on isoenzyme of esterase (EST) and dehydrogenase (LDH) in adults and larvae of Chinese and American Trichinella spiralis (T.s) by PAGE and UVP gel documentation analysis system were reported. The results showed that there were 2 bands of EST isoenzyme in adults and larvae of Chinese heilongjian T.s , respectively. There was only one band of LDH in Chinese T.s larvae. In EST analysis 3 and 2 enzymatic bands were found in adults and larvae of American T.s , respectively. No LDH isoenzyme band of were observed in adults of Chinese and American T.s . Otherwise one band was found both in Chinese larvae and American larvae, the relative mobility was also different. The results indicated that there was genetic difference in different geographical T.s strains. In addition, there was great difference in isoenzyme patterns of different developmental stages of T.s .

Key words: different developmental stages of T.s, isoenzyme of esterase(EST), isoenzyme of dehydrogenase (LDH)

中图分类号:

R383.1⁺⁵

张帆;姜洪杰;黄松;诸欣平. 中美猪型旋毛虫株不同发育阶段同工酶的电泳分析[J]. 首都医科大学学报, 2000, 21(1): 41-42.

Zhang Fan;Jiang Hongjie;Huang Song;Zhu Xinping. Electrophoresis Analysis on Isoenzyme of Different Development Stages of Chinese and American Trichinella spiralis[J]. Journal of Capital Medical University, 2000, 21(1): 41-42.