成年大鼠心肌细胞培养方法的建立和形态学观察

首都医科大学学报 ›› 2000, Vol. 21 ›› Issue (2): 104-107.

成年大鼠心肌细胞培养方法的建立和形态学观察

许秀芳¹, 李温斌², 陈宝田², 吕燕宁¹, 赵莉敏¹, 陈燕¹

1. 北京心肺血管疾病研究所细胞免疫室;2. 首都医科大学附属北京安贞医院心外科

收稿日期:1999-03-09 修回日期:1900-01-01 出版日期:2000-04-15 发布日期:2000-04-15

Morphology Study and Isolated and Cultured Method of Adult Myocytes

Xu Xiufang¹, Li Wenbin², Chen Baotian², Lü Yanning¹, Zhao Limin¹, Chen Yan¹

1. Beijing Heart Lung and Blood Vessel Institute;2. Department of Heart Surgery of Beijing Anzhen Hospital, Affiliate of Capital University of Medical Sciences

Received:1999-03-09 Revised:1900-01-01 Online:2000-04-15 Published:2000-04-15

摘要/Abstract

摘要： 为探索稳定的成年大鼠心肌细胞的分离和培养方法,应用生物酶(5g/L胰蛋白酶及1g/L胶原酶)灌注法分离成年大鼠心肌细胞,用形态学及台盼蓝染色法鉴定分离的心肌细胞,并在光镜和电镜下观察和摄像记录。结果：心肌细胞的存活率为91.7%;存活的心肌细胞静止地贴在培养板底上,细胞完整,呈杆状,长宽比约为4～6:1.结果提示：该方法是比较理想的心肌细胞培养法。

关键词: 成年大鼠心肌细胞, 胰蛋白酶, 胶原酶, 分离技术

Abstract: In order to build method of a stable isolation and culture of adult myocytes, adult rat myocytes were isolated with bioenzymes (including 5 g/L trypsin and 1 g/L colleagase) perfusing tissue through acceding aorta of the heart. Calcium-tolerant myocytes were harvested, purifyed and cultured with DMEM culture medium . The myocytes quality was evaluated with morphology and trypan blue dye. States of myocytes growing in culture were observed with optical microscope and electron microscope photography. Result: Survival rate of aduld rat myocytes was 91.7%, survival myocytes were motionlessly attached to the bottom of culture plates, they were intact and rod-shaped, their length/width ratio was 4～6: 1. Conclusion: The method of isolation and culture of adult rat myocytes was successful.

Key words: adult rat myocytes, trypsin, colleagase, separation technique

中图分类号:

许秀芳;李温斌;陈宝田;吕燕宁;赵莉敏;陈燕. 成年大鼠心肌细胞培养方法的建立和形态学观察[J]. 首都医科大学学报, 2000, 21(2): 104-107.

Xu Xiufang;Li Wenbin;Chen Baotian;Lü Yanning;Zhao Limin;Chen Yan. Morphology Study and Isolated and Cultured Method of Adult Myocytes[J]. Journal of Capital Medical University, 2000, 21(2): 104-107.

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