人IgG1FccDNA的克隆与鉴定

首都医科大学学报 ›› 2000, Vol. 21 ›› Issue (2): 93-96.

人IgG1FccDNA的克隆与鉴定

安云庆, 柯岩, 陈金栋

首都医科大学微生物学和免疫学教研室

收稿日期:1999-08-27 修回日期:1900-01-01 出版日期:2000-04-15 发布日期:2000-04-15

Cloning and Identification of Human IgG1Fc cDNA

An Yunqing, Ke Yan, Chen Jindong

Department of Microbiology and Immunology, Capital University of Medical Sciences

Received:1999-08-27 Revised:1900-01-01 Online:2000-04-15 Published:2000-04-15

摘要/Abstract

摘要： 根据编码人IgG1Fc(Fcγ1)的基因序列,设计合成1对与之相匹配的引物。采用RT-PCR法,从正常人白细胞mRNA中扩增获得预期目的基因——Fcγ1_700bp基因片段,成功构建PUC18-Fcγ1₇₀₀重组克隆载体,酶切、酶谱分析与预期结果相符;DNA测序结果与GenBank报道一致。

关键词: IgG1Fc(Fcγ1), PUC18-Fcγ1₇₀₀重组克隆载体, RT-PCR

Abstract: According to the cDNA sequences which encode the human IgG1Fc, a pair of primers were designed; The expected gene (Fcγ1_700bp) which encode Fcγ1 was amplified by RT-PCR from mRNA extracted from normal person′s leukocyte; PUC18-Fcγ1₇₀₀ recombinant cloning vector was successfully constructed, and the enzyme digestion analysis are identical to expected results. Sequences are identical to those in GenBank report.

Key words: IgG1Fcgene(Fcγ1_700bp), PUC18-Fcγ1₇₀₀ recombinant cloning vector, RT-PCR

中图分类号:

R392.1

安云庆;柯岩;陈金栋. 人IgG1FccDNA的克隆与鉴定[J]. 首都医科大学学报, 2000, 21(2): 93-96.

An Yunqing;Ke Yan;Chen Jindong. Cloning and Identification of Human IgG1Fc cDNA[J]. Journal of Capital Medical University, 2000, 21(2): 93-96.

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