急性缺氧对大鼠血管内皮细胞的损伤

首都医科大学学报 ›› 2004, Vol. 25 ›› Issue (2): 189-192.

急性缺氧对大鼠血管内皮细胞的损伤

王岸柳¹, 温祥云², 刘国贞²

1. 首都医科大学附属北京天坛医院病理科;2. 首都医科大学病理学教研室

收稿日期:2003-08-05 修回日期:1900-01-01 出版日期:2004-04-15 发布日期:2004-04-15

Vascular Endothelial Cells Injury After Acute Hypoxia

Wang Anliu¹, Wen Xiangyun², Liu Guozhen²

1. Department of Pathology, Beijing Tiantan Hospital, Affiliate of Capital University of Medical Sciences;2. Department of Pathology, Capital University of Medical Sciences

Received:2003-08-05 Revised:1900-01-01 Online:2004-04-15 Published:2004-04-15

摘要/Abstract

摘要： 应用光镜及电镜,对急性缺氧状态下大鼠的肺小动脉内皮细胞(pulmonary small artery endothelial cell,PSAEC)及肺毛细血管内皮细胞(pulmonary capillary endothelial cell,PCEC)进行形态学观察,并用显微分光光度仪对急性缺氧状态下PSAEC的乳酸脱氢酶(lactic dehydrogenase,LDH)活性进行定量分析。发现:急性缺氧可引起PSAEC和PCEC发生各种损伤性形态变化,损伤程度随缺氧时间延长而加重;与缺氧前相比,急性缺氧后各组PSAEC的LDH活性均下降,且随缺氧时间的延长,LDH活性下降得更明显。提示:急性缺氧可引起血管内皮细胞(VEC)损伤,损伤程度随缺氧时间的延长而加重,血管内皮细胞损伤的程度与其LDH活性密切相关。

关键词: 急性缺氧, 血管内皮细胞, 乳酸脱氢酶

Abstract: Pathological changes of pulmonary small artery endothelial cells and pulmonary capillary endothelial cells under hypoxia were observed morphologically by opticalmicroscope and electromicroscope respectively, and LDH activity of pulmonary small artery endothelial under hypoxia was analyzed by histochemical staining image. Injure changes resulted from acute hypoxia, LDH activity of pulmonary small artery endothelial in groups after hypoxia was lower than that in groups before hypoxia and decreased progressively with hypoxia time. Acute hypoxia may injury vascular endothelial cells and the injury aggravates with hypoxia time, the extent to which the vascular endothelial cell is injuried is closely related to the LDH activity of it.

Key words: acute hypoxia, vascular endothelial cell, lactic dehydrogenase

中图分类号:

王岸柳;温祥云;刘国贞. 急性缺氧对大鼠血管内皮细胞的损伤[J]. 首都医科大学学报, 2004, 25(2): 189-192.

Wang Anliu;Wen Xiangyun;Liu Guozhen. Vascular Endothelial Cells Injury After Acute Hypoxia[J]. Journal of Capital Medical University, 2004, 25(2): 189-192.

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